| Extracellular haemoglobin, oxidative stress and quality of red blood cells relative to perioperative blood salvage. | |
| | |
MedLine Citation:
|
PMID: 20158444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The quality of blood-pack units in perioperative blood salvage was studied with respect to the presence of extracellular haemoglobin (Hb) and in terms of oxidative stress and the mechanical properties of red blood cells (RBC). METHODS: The study was performed on blood-pack units and patients' blood after retransfusion. Results were compared to those obtained in patients prior to autotransfusion. Free Hb, non-protein thiols (as antioxidant marker) and markers of oxidative stress (conjugated dienes, thiobarbituric reactive substances and protein carbonyl content) were measured. The mechanical properties of RBC were evaluated by their osmotic fragility, membrane fluidity by measuring the fluorescence anisotropy of an extrinsic probe and permeability by measurement of extracellular K(+) and lactate dehydrogenase. RESULTS: Despite washing, extracellular Hb concentrations remained high (up to 0.7 g/L in blood-pack units) and was associated with a decrease of haptoglobin in patients, despite a concomitant inflammatory syndrome. In blood-pack units, we observed a decrease in antioxidant markers along with an increase in oxidative stress markers in association with an alteration of RBC membrane properties. CONCLUSIONS: Haemolysis must be limited during perioperative blood salvage in order to prevent exposure to oxidative stress and improve the efficiency of autotransfusion. |
| | |
Authors:
|
Papa Madieye Gueye; Françoise Bertrand; Guy Duportail; Jean-Marc Lessinger |
Related Documents
:
|
3335994 - Role of dapsone hydroxylamine in dapsone-induced hemolytic anemia. 6602664 - Use of turkey red blood cells in the passive haemagglutination test for studying tetanu... 9369814 - The effect of the source of transfused blood on the rate of consumption of transfused r... 4612134 - The dispersion of solutes during blood flow through a straight tube. 2709864 - The effects of deep hypothermic cardiopulmonary bypass and total circulatory arrest on ... 595114 - Causes of disqualification in a volunteer blood donor population. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-05-10 Completed Date: 2010-07-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
|
Languages: eng Pagination: 677-83 Citation Subset: IM |
Affiliation:
|
Laboratoire de Biochimie Pharmaceutique, Centre Hospitalier Universitaire National de Fann, Université Cheikh Anta Diop, Dakar, Sénégal. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aged Aged, 80 and over Antioxidants / metabolism Biological Markers / blood Blood Preservation Blood Transfusion, Autologous / methods* Erythrocyte Membrane / metabolism, physiology Erythrocytes / chemistry, cytology, physiology* Haptoglobins / analysis Hemoglobins / analysis* Hemolysis Humans Membrane Fluidity Middle Aged Osmotic Fragility Oxidative Stress* Stress, Mechanical Sulfhydryl Compounds / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Biological Markers; 0/Haptoglobins; 0/Hemoglobins; 0/Sulfhydryl Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cardiovascular risk factors and gamma-glutamyltransferase fractions in healthy individuals.
Next Document: A new statistical method for evaluating long-term analytical performance of laboratories applied to ...