Document Detail

Extracellular dextranase from Streptococcus oralis.
MedLine Citation:
PMID:  1470049     Owner:  NLM     Status:  MEDLINE    
A human dental plaque organism, Streptococcus oralis (S. mitior), was cultivated in a dextran-free, dialysed medium, and dextranase activity was isolated from the cell-free, culture supernatant. The lyophilized, crude enzyme preparation, optimum pH 6, was subjected sequentially to anion exchange and gel filtration fast protein liquid chromatography (FPLC). The dextranolytic fraction from gel filtration FPLC produced a symmetrical, baseline resolved peak. The dextranolytic enzyme was purified 1,126-fold with a yield of 2.4%. Amino acid analysis revealed a large proportion of alanine and an abundance of acidic amino acids. This extracellular enzyme isolated from S. oralis is constitutive and has a relative molecular mass of 45 kD. Further investigation of the possible structural and biochemical effects of endogenous bacterial glucanases in human dental plaques is necessary.
I H Johnson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbios     Volume:  72     ISSN:  0026-2633     ISO Abbreviation:  Microbios     Publication Date:  1992  
Date Detail:
Created Date:  1993-01-26     Completed Date:  1993-01-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0207257     Medline TA:  Microbios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  35-45     Citation Subset:  IM    
School of Dental Science, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Victoria, Australia.
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MeSH Terms
Amino Acids / analysis
Chromatography, Liquid
Dextranase / chemistry,  isolation & purification*
Molecular Weight
Streptococcus / enzymology*
Reg. No./Substance:
0/Amino Acids; EC

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