Document Detail


Extracellular dextranase from Streptococcus oralis.
MedLine Citation:
PMID:  1470049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human dental plaque organism, Streptococcus oralis (S. mitior), was cultivated in a dextran-free, dialysed medium, and dextranase activity was isolated from the cell-free, culture supernatant. The lyophilized, crude enzyme preparation, optimum pH 6, was subjected sequentially to anion exchange and gel filtration fast protein liquid chromatography (FPLC). The dextranolytic fraction from gel filtration FPLC produced a symmetrical, baseline resolved peak. The dextranolytic enzyme was purified 1,126-fold with a yield of 2.4%. Amino acid analysis revealed a large proportion of alanine and an abundance of acidic amino acids. This extracellular enzyme isolated from S. oralis is constitutive and has a relative molecular mass of 45 kD. Further investigation of the possible structural and biochemical effects of endogenous bacterial glucanases in human dental plaques is necessary.
Authors:
I H Johnson
Related Documents :
23850559 - Oxidized fatty acid analysis by charge switch derivatization, selected reaction monitor...
24419699 - Apical dominance and the levels of indole acetic acid in phaseolus lateral buds.
3130169 - The carbohydrate moiety of mineral-bound proteins from fetal enamel: a basis for enamel...
23180549 - Heterologous expression and biochemical characterization of acetyl xylan esterase from ...
3947079 - Isolation and characterization of chum salmon growth hormone.
7787299 - Rapid and simple procedure for purifying pas-4 glycoprotein from bovine milk fat globul...
8589609 - Classification of amino acid spin systems using pfg hcc(co)nh-tocsy with constant-time ...
24224439 - The protective effect of ellagic acid on lung damage caused by experimental obstructive...
9675139 - Positional specificity of rabbit cyp4b1 for omega-hydroxylation1 of short-medium chain ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbios     Volume:  72     ISSN:  0026-2633     ISO Abbreviation:  Microbios     Publication Date:  1992  
Date Detail:
Created Date:  1993-01-26     Completed Date:  1993-01-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0207257     Medline TA:  Microbios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  35-45     Citation Subset:  IM    
Affiliation:
School of Dental Science, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Victoria, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Chromatography, Liquid
Dextranase / chemistry,  isolation & purification*
Molecular Weight
Streptococcus / enzymology*
Chemical
Reg. No./Substance:
0/Amino Acids; EC 3.2.1.11/Dextranase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Nutritional and physiological factors affecting germination of heterothallic Saccharomyces cerevisia...
Next Document:  Sensitivity of Pseudomonas stutzeri to EDTA: effects of growth parameters and test conditions.