Document Detail


Extracellular cAMP is sufficient to restore developmental gene expression and morphogenesis in Dictyostelium cells lacking the aggregation adenylyl cyclase (ACA).
MedLine Citation:
PMID:  8224844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell movement and cell-type-specific gene expression during Dictyostelium development are regulated by cAMP, which functions both as an extracellular hormone-like signal and an intracellular second messenger. Previous data indicated that aca- mutants, which lack adenylyl cyclase activity, fail to aggregate and do not express cell-type-specific genes. We show here that overexpression of ACG, a constitutively active adenylyl cyclase, which in wild-type cells is only expressed during spore germination, partially restores the coordination of cell movement and completely restores developmental gene expression. The aca- cells can also be induced to develop into viable spores by synergy with wild-type cells and, furthermore, form small but normal fruiting bodies, after a developmentally relevant regimen of stimulation with nanomolar cAMP pulses followed by micromolar cAMP concentrations. 2'-Deoxy cAMP, a cAMP analog that activates the cell-surface cAMP receptors but not cAMP-dependent protein kinase (PKA), also induces fruiting body formation as well as expression of prespore-specific and prestalk-enriched genes in aca- cells. Intracellular cAMP levels were not altered in aca- cells after stimulation with 2'-deoxy cAMP. Our data indicate that ACA is not required to provide intracellular cAMP for PKA activation but is essential to produce extracellular cAMP for coordination of cell movement during all stages of development and for induction of developmental gene expression.
Authors:
G S Pitt; R Brandt; K C Lin; P N Devreotes; P Schaap
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genes & development     Volume:  7     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-12-16     Completed Date:  1993-12-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2172-80     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / genetics*
Animals
Cyclic AMP / analogs & derivatives,  metabolism,  pharmacology*
Cyclic GMP / metabolism
Dictyostelium / cytology,  genetics*,  growth & development*
Gene Deletion
Gene Expression Regulation, Fungal* / drug effects
Genes, Fungal
Kinetics
Morphogenesis / drug effects
RNA, Messenger / analysis,  biosynthesis
Spores, Fungal / physiology
Time Factors
Chemical
Reg. No./Substance:
0/RNA, Messenger; 1157-33-1/2'-deoxy cyclic AMP; 60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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