Document Detail


Extracellular ascorbic acid fluctuation during the protective process of ischemic preconditioning in rabbit renal ischemia-reperfusion model measured.
MedLine Citation:
PMID:  20819604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Ascorbic acid has important antioxidant properties, and may play a role in the protective effects of ischemic preconditioning on later ischemia-reperfusion. Herein, we examined the role of endogenous extracellular ascorbic acid in ischemic preconditioning in the kidney.
METHODS: We developed a solitary rabbit kidney model where animals received ischemia-reperfusion only (ischemia-reperfusion group, n = 15) or ischemic preconditioning followed by ischemia-reperfusion (ischemic preconditioning group, n = 15). Ischemia-reperfusion was induced by occluding and loosening of the renal pedicle. The process of ischemic preconditioning included 15-minute brief ischemia and 10-minute reperfusion. In vivo microdialysis coupled with online electrochemical detection was used to determine levels of endogenous extracellular ascorbic acid in both groups. The extent of tissue damage was determined in kidney sections stained with hematoxylin and eosin. Serum creatinine and urea nitrogen were also detected to assess renal function.
RESULTS: During ischemia-reperfusion, the extracellular ascorbic acid concentration during ischemia increased rapidly to the peak level ((130.01 +/- 9.98)%), and then decreased slowly to near basal levels. Similar changes were observed during reperfusion (peak level, (126.78 +/- 18.24)%). In the ischemic preconditioning group there was a similar pattern of extracellular ascorbic acid concentration during ischemic preconditioning. However, the ascorbic acid level was significantly lower during the ischemia and early reperfusion stage compared to the ischemia-reperfusion group. Additionally, the extent of glomerular ischemic collapse, tubular dilation, tubular denudation, and loss of brush border were markedly attenuated in the ischemic preconditioning group. Levels of serum creatinine and urea nitrogen were also decreased significantly in the ischemic preconditioning group.
CONCLUSIONS: Ischemic preconditioning may protect renal tissue against ischemia-reperfusion injury via use of extracellular ascorbic acid. In vivo microdialysis coupled with online electrochemical detection is effective for continuous monitoring extracellular ascorbic acid in the renal cortex.
Authors:
Lei Liu; Yu-qing Lin; Long-tao Yan; Kai Hong; Xiao-fei Hou; Lan-qun Mao; Lu-lin Ma
Related Documents :
3956234 - The alteration of lipid peroxide in glucocorticoid-induced cataract of developing chick...
9185034 - Microplate assay of ascorbic acid in aqueous humor with bicinchoninic acid.
21144 - The effect of additives on the free radical formation in aqueous solutions of ascorbic ...
7066604 - Differential pulse voltammetry in the anaesthetized rat: identification of ascorbic aci...
20060014 - Phomenins and fatty acids from alternaria infectoria.
3731214 - The effects of short-chain fatty acids on the neuronal membrane functions of helix poma...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  123     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  1441-6     Citation Subset:  IM    
Affiliation:
Department of Urology, Peking University Third Hospital, Beijing 100191, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / metabolism*
Disease Models, Animal
Ischemic Preconditioning / methods*
Kidney / metabolism*,  pathology
Rabbits
Reperfusion Injury / prevention & control*
Chemical
Reg. No./Substance:
50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Study of an integrated non-motor symptoms questionnaire for Parkinson's disease.
Next Document:  Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion ...