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Extracellular acidic pH-activated, outward rectifying chloride currents can be regulated by reactive oxygen species in human THP-1 monocytes.
MedLine Citation:
PMID:  23376719     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Extracellular acidic pH-activated chloride channels (I(Cl, acid)) have been found in a variety of mammalian cells. In the present study, the expression and regulation of I(Cl,acid) were investigated in THP-1 cells. Patch clamp recordings demonstrated that an extracellular acidic solution induced an outward rectified current, which could be blocked by the Cl(-) channel blocker. The currents exhibited time-dependent facilitation and inactivation. The relative anion permeability of this current followed the sequence Cl(-)> Br(-)> I(-)>gluconate. NADPH oxidase inhibitors did not decrease pH 4.4 -induced currents. However, reactive oxygen species (ROS) scavengers and mitochondrial inhibitors inhibited pH 4.4 -induced currents. Fluorescence imaging of intracellular ROS and mitochondrial activity confirmed these findings. We conclude that I(Cl,acid) occurs in human THP-1 cells and that I(Cl,acid) may be regulated by intracellular ROS mainly originating from mitochondria.
Authors:
Zhi-Jie Fu; Xue-Zhong Li; Qi-Rong Wang; Li Shi; Li-Qiang Zhang; Xin-Liang Pan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-31
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier Inc.
Affiliation:
Department of Otorhinolaryngology, QiLu Hospital, Shandong University, Jinan, China.
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