| Extracellular acidic pH-activated, outward rectifying chloride currents can be regulated by reactive oxygen species in human THP-1 monocytes. | |
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MedLine Citation:
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PMID: 23376719 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Extracellular acidic pH-activated chloride channels (I(Cl, acid)) have been found in a variety of mammalian cells. In the present study, the expression and regulation of I(Cl,acid) were investigated in THP-1 cells. Patch clamp recordings demonstrated that an extracellular acidic solution induced an outward rectified current, which could be blocked by the Cl(-) channel blocker. The currents exhibited time-dependent facilitation and inactivation. The relative anion permeability of this current followed the sequence Cl(-)> Br(-)> I(-)>gluconate. NADPH oxidase inhibitors did not decrease pH 4.4 -induced currents. However, reactive oxygen species (ROS) scavengers and mitochondrial inhibitors inhibited pH 4.4 -induced currents. Fluorescence imaging of intracellular ROS and mitochondrial activity confirmed these findings. We conclude that I(Cl,acid) occurs in human THP-1 cells and that I(Cl,acid) may be regulated by intracellular ROS mainly originating from mitochondria. |
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Authors:
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Zhi-Jie Fu; Xue-Zhong Li; Qi-Rong Wang; Li Shi; Li-Qiang Zhang; Xin-Liang Pan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-31 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2013. Published by Elsevier Inc. |
Affiliation:
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Department of Otorhinolaryngology, QiLu Hospital, Shandong University, Jinan, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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