Document Detail

Extracellular H+ stimulates the expression of c-fos/c-jun mRNA through Ca2+/calmodulin in PC12 cells.
MedLine Citation:
PMID:  9754718     Owner:  NLM     Status:  MEDLINE    
Evidence has accumulated that an increase in extracellular protons stimulates the transmembrane mechanism to induce various intracellular responses, such as the expression of c-fos and c-jun. In the present study, we aimed to obtain evidence that an increase in extracellular protons induces expression of c-fos/c-jun mRNA in PC12 pheochromocytoma cells of rats. We found that the c-fos/c-jun mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH values and the expression of c-fos/c-jun mRNA. To determine whether the Ca2+/calmodulin system subserves the H+-induced expression of c-fos/c-jun, Ca2+/calmodulin inhibitor trifluoperazine was added to PC12 cells. We found that trifluoperazine inhibited the expression of the H+-induced c-fos/c-jun mRNA by 30-35%. In contrast, trifluoperazine did not inhibit the expression of phorbol-induced c-fos/c-jun mRNA. These results indicate that an increase in extracellular protons induces the expression of c-fos/c-jun mRNA, and this expression is mediated partly by the Ca2+/calmodulin system.
N Shimokawa; S Sugama; M Miura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  10     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1999-01-13     Completed Date:  1999-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  499-503     Citation Subset:  IM    
Department of Physiology 1st Division, Gunma University School of Medicine, Maebashi City, Japan.
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MeSH Terms
Calcium / metabolism*
Calmodulin / metabolism*
Extracellular Space
Gene Expression Regulation*
PC12 Cells
Proto-Oncogene Proteins c-fos / genetics*
Proto-Oncogene Proteins c-jun / genetics*
Reg. No./Substance:
0/Calmodulin; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 1333-74-0/Hydrogen; 7440-70-2/Calcium

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