| Extracellular GAPDH binds to L1 and enhances neurite outgrowth. | |
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MedLine Citation:
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PMID: 19285135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a binding partner for the cell adhesion molecule L1. GAPDH binds to sites within the extracellular domain of L1, namely the immunoglobulin-like domains I-VI and the fibronectin type III homologous repeats 4-5. Extracellular GAPDH was detected at the cell surface of neuronal cells by surface biotinylation and immunocytochemistry. Addition of GAPDH antibodies to cultured cerebellar neurons inhibited L1-dependent neurite outgrowth in the presence of ATP, while the application of exogenous GAPDH promoted L1-dependent neurite outgrowth. Pre-treatment of substrate-coated L1-Fc with ATP and GAPDH, which phosphorylates L1, subsequently led to an enhanced neurite outgrowth. Furthermore, aggregation of L1-Fc carrying beads was enhanced in the presence of both GAPDH and ATP. L1-dependent neurite outgrowth and aggregation of L1 were diminished in the presence of alkaline phosphatase or a protein kinase inhibitor. Our results show that GAPDH-dependent phosphorylation of L1 is a novel mechanism in regulating L1-mediated neurite outgrowth. |
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Authors:
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Tatjana Makhina; Gabriele Loers; Christian Schulze; Barbara Ueberle; Melitta Schachner; Ralf Kleene |
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Publication Detail:
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Type: Journal Article Date: 2009-03-11 |
Journal Detail:
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Title: Molecular and cellular neurosciences Volume: 41 ISSN: 1095-9327 ISO Abbreviation: Mol. Cell. Neurosci. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-25 Completed Date: 2009-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100095 Medline TA: Mol Cell Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 206-18 Citation Subset: IM |
Affiliation:
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Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, 20246 Hamburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Brain Neoplasms Cell Line, Tumor Cerebellum / cytology Glyceraldehyde-3-Phosphate Dehydrogenases / genetics, metabolism* Humans Immunoglobulin Fc Fragments / genetics, metabolism Mice Mice, Inbred C57BL Neural Cell Adhesion Molecule L1 / genetics, metabolism* Neural Cell Adhesion Molecules / genetics, metabolism Neurites / physiology* Neuroblastoma Neurons* / cytology, metabolism Protein Binding Protein Structure, Tertiary Rats |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Fc Fragments; 0/Neural Cell Adhesion Molecule L1; 0/Neural Cell Adhesion Molecules; 56-65-5/Adenosine Triphosphate; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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