Document Detail


Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions.
MedLine Citation:
PMID:  20539754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptococcus gattii, an emerging fungal pathogen of humans and animals, is found on a variety of trees in tropical and temperate regions. The ecological niche and virulence of this yeast remain poorly defined. We used Arabidopsis thaliana plants and plant-derived substrates to model C. gattii in its natural habitat. Yeast cells readily colonized scratch-wounded plant leaves and formed distinctive extracellular fibrils (40-100 nm diameter x500-3000 nm length). Extracellular fibrils were observed on live plants and plant-derived substrates by scanning electron microscopy (SEM) and by high voltage- EM (HVEM). Only encapsulated yeast cells formed extracellular fibrils as a capsule-deficient C. gattii mutant completely lacked fibrils. Cells deficient in environmental sensing only formed disorganized extracellular fibrils as apparent from experiments with a C. gattii STE12alpha mutant. C. gattii cells with extracellular fibrils were more virulent in murine model of pulmonary and systemic cryptococcosis than cells lacking fibrils. C. gattii cells with extracellular fibrils were also significantly more resistant to killing by human polymorphonuclear neutrophils (PMN) in vitro even though these PMN produced elaborate neutrophil extracellular traps (NETs). These observations suggest that extracellular fibril formation could be a structural adaptation of C. gattii for cell-to-cell, cell-to-substrate and/or cell-to- phagocyte communications. Such ecological adaptation of C. gattii could play roles in enhanced virulence in mammalian hosts at least initially via inhibition of host PMN- mediated killing.
Authors:
Deborah J Springer; Ping Ren; Ramesh Raina; Yimin Dong; Melissa J Behr; Bruce F McEwen; Samuel S Bowser; William A Samsonoff; Sudha Chaturvedi; Vishnu Chaturvedi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-07
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-09-01     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e10978     Citation Subset:  IM    
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, New York, United States of America.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / microbiology
Cryptococcus gattii / pathogenicity*,  physiology,  ultrastructure
Disease Models, Animal
Humans
Male
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Neutrophils / microbiology*
Plant Leaves / microbiology
Virulence
Grant Support
ID/Acronym/Agency:
AI-48462/AI/NIAID NIH HHS; AI-53732/AI/NIAID NIH HHS; P41-RR01219/RR/NCRR NIH HHS
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