Document Detail

Extracellular carbohydrate esterase from the basidiomycete Coprinopsis cinerea released ferulic and acetic acids from xylan.
MedLine Citation:
PMID:  20699557     Owner:  NLM     Status:  MEDLINE    
cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase.
Kohsuke Hashimoto; Satoshi Kaneko; Makoto Yoshida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  74     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-12-15     Revised Date:  2010-12-28    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1722-4     Citation Subset:  IM    
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo, Japan.
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MeSH Terms
Acetic Acid / metabolism*
Agaricales / cytology*,  enzymology*,  metabolism
Amino Acid Sequence
Cloning, Molecular
Coumaric Acids / metabolism*
Esterases / chemistry,  genetics,  metabolism*
Extracellular Space / enzymology*
Molecular Sequence Data
Xylans / metabolism*
Reg. No./Substance:
0/Coumaric Acids; 0/Xylans; 1135-24-6/ferulic acid; 64-19-7/Acetic Acid; EC 3.1.-/Esterases

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