| Extracellular carbohydrate esterase from the basidiomycete Coprinopsis cinerea released ferulic and acetic acids from xylan. | |
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MedLine Citation:
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PMID: 20699557 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase. |
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Authors:
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Kohsuke Hashimoto; Satoshi Kaneko; Makoto Yoshida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 74 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-25 Completed Date: 2010-12-15 Revised Date: 2010-12-28 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1722-4 Citation Subset: IM |
Affiliation:
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Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB540992 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism* Agaricales / cytology*, enzymology*, metabolism Amino Acid Sequence Cloning, Molecular Coumaric Acids / metabolism* Esterases / chemistry, genetics, metabolism* Extracellular Space / enzymology* Molecular Sequence Data Xylans / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Coumaric Acids; 0/Xylans; 1135-24-6/ferulic acid; 64-19-7/Acetic Acid; EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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