Document Detail


External pH influences the transcriptional profile of the carbonic anhydrase, CAH-4b in Caenorhabditis elegans.
MedLine Citation:
PMID:  18640159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Insight into how organisms adapt to environmental stimuli has become increasingly important in recent years for identifying key virulence factors in many species. The life cycle of many pathogenic nematode species forces the organism to experience environments which would otherwise be considered stressful. One of the conditions often encountered by nematodes is a change in environmental pH. Living in a soil environment Caenorhabditis elegans will naturally encounter fluctuations in external pH. Therefore, C. elegans has the potential to provide an insight into how pathogenic nematodes survive and proliferate in these environments. We found that C. elegans can maintain over 90% survival in pH conditions ranging from pH 3 to 10. This was unrelated to the non-specific protection provided by the cuticle. Global transcriptional analysis identified many genes, which were differentially regulated by pH. The gene cah-4 encodes two putative alpha carbonic anhydrases (CAH-4a and CAH-4b), one of which was five-fold up regulated in an alkaline environment (CAH-4b). Stopped-flow analysis of CAH-4b using 35 different carbonic anhydrase inhibitors identified complex benzenesulfonamide compounds as the most potent inhibitors (K(i) 35-89nM).
Authors:
Rebecca A Hall; Daniela Vullo; Alessio Innocenti; Andrea Scozzafava; Claudiu T Supuran; Peter Klappa; Fritz A Mühlschlegel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-03
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  161     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-08-18     Completed Date:  2008-10-23     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  140-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / genetics,  growth & development,  metabolism*
Caenorhabditis elegans Proteins / genetics,  metabolism*
Carbonic Anhydrases / genetics,  metabolism*
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Hydrogen-Ion Concentration
Oligonucleotide Array Sequence Analysis
Up-Regulation
Grant Support
ID/Acronym/Agency:
G0601049//Medical Research Council; //Biotechnology and Biological Sciences Research Council; //Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; EC 4.2.1.1/Carbonic Anhydrases

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