Document Detail

Extensive post-translational modification, including serine to D-alanine conversion, in the two-component lantibiotic, lacticin 3147.
MedLine Citation:
PMID:  10608807     Owner:  NLM     Status:  MEDLINE    
Lacticin 3147 is a two-component bacteriocin produced by Lactococcus lactis subspecies lactis DPC3147. In order to further characterize the biochemical nature of the bacteriocin, both peptides were isolated which together are responsible for the antimicrobial activity. The first, LtnA1, is a 3,322 Da 30-amino acid peptide and the second component, LtnA2, is a 29-amino acid peptide with a mass of 2,847 Da. Conventional amino acid analysis revealed that both peptides contain the thioether amino acid, lanthionine, as well as an excess of alanine to that predicted from the genetic sequence of the peptides. Chiral phase gas chromatography coupled with mass spectrometry of amino acid composition indicated that both LtnA1 and LtnA2 contain D-alanine residues and amino acid sequence analysis of LtnA1 confirmed that the D-alanine results from post-translational modification of a serine residue in the primary translation product. Taken together, these results demonstrate that lacticin 3147 is a novel, two-component, D-alanine containing lantibiotic that undergoes extensive post-translational modification which may account for its potent antimicrobial activity against a wide range of Gram-positive bacteria.
M P Ryan; R W Jack; M Josten; H G Sahl; G Jung; R P Ross; C Hill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-08     Completed Date:  2000-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  37544-50     Citation Subset:  IM    
Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
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MeSH Terms
Alanine / chemistry*
Amino Acid Sequence
Amino Acid Substitution
Anti-Bacterial Agents / chemistry,  isolation & purification,  metabolism*
Bacterial Proteins / chemistry,  isolation & purification,  metabolism
Chromatography, High Pressure Liquid
Mass Spectrometry
Molecular Sequence Data
Protein Processing, Post-Translational*
Serine / chemistry*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Bacteriocins; 136959-47-2/lacticin 481; 56-41-7/Alanine; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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