Document Detail

Extensive chromosome rearrangements distinguish the karyotype of the hypovirulent species Candida dubliniensis from the virulent Candida albicans.
MedLine Citation:
PMID:  17719250     Owner:  NLM     Status:  MEDLINE    
Candida dubliniensis and Candida albicans, the most common human fungal pathogen, have most of the same genes and high sequence similarity, but C. dubliniensis is less virulent. C. albicans causes both mucosal and hematogenously disseminated disease, C. dubliniensis mostly mucosal infections. Pulse-field electrophoresis, genomic restriction enzyme digests, Southern blotting, and the emerging sequence from the Wellcome Trust Sanger Institute were used to determine the karyotype of C. dubliniensis type strain CD36. Three chromosomes have two intact homologues. A translocation in the rDNA repeat on chromosome R exchanges telomere-proximal regions of R and chromosome 5. Translocations involving the remaining chromosomes occur at the Major Repeat Sequence. CD36 lacks an MRS on chromosome R but has one on 3. Of six other C. dubliniensis strains, no two had the same electrophoretic karyotype. Despite extensive chromosome rearrangements, karyotypic differences between C. dubliniensis and C. albicans are unlikely to affect gene expression. Karyotypic instability may account for the diminished pathogenicity of C. dubliniensis.
B B Magee; Melissa D Sanchez; David Saunders; David Harris; M Berriman; P T Magee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-07-20
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  45     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-03-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  338-50     Citation Subset:  IM    
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, USA.
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MeSH Terms
Antigens, CD36 / genetics
Blotting, Southern
Candida / classification,  genetics*,  pathogenicity
Candida albicans / classification,  genetics*,  pathogenicity
Chromosome Aberrations*
Chromosomes, Fungal / genetics*
DNA, Fungal / genetics
DNA, Ribosomal / genetics
Electrophoresis, Gel, Pulsed-Field
Karyotyping / methods
Mycological Typing Techniques
Grant Support
AI16567/AI/NIAID NIH HHS; R01 AI016567-21/AI/NIAID NIH HHS; R01 AI016567-22/AI/NIAID NIH HHS; R01 AI016567-23/AI/NIAID NIH HHS; //Wellcome Trust
Reg. No./Substance:
0/Antigens, CD36; 0/DNA, Fungal; 0/DNA, Ribosomal

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