| Extensive apoptosis and abnormal morphogenesis in pro-caspase-3 transgenic zebrafish during development. | |
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MedLine Citation:
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PMID: 18515717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pro-apoptotic caspase-3 gene has been shown to have key functions in the execution of apoptosis (programmed cell death) in vertebrate cells. However, the central role of caspase-3 in morphogenesis during development remains unclear. In this study, transgenic zebrafish that overexpress full-length pro-caspase-3 were generated to determine the effects of caspase genes on vertebrate morphogenesis and stress tolerance. The enhanced expression of the full-length pro-caspase-3 cDNA induced extremely high levels of caspase activity and extensive apoptosis in the transgenic embryos, and 33-46% of F2 embyos in the transgenic lines exhibited some form of morphological abnormality. Pro-caspase-3 transgenic zebrafish exhibited abnormal morphogenesis in the eyes, notochord, heart and yolk sac, suggesting that enhanced processing of pro-caspase-3 triggers significant apoptotic responses in the specific target tissues that are undergoing morphogenesis during development. The transgenic fish had reduced eye size and showed degeneration of the retina, including the photoreceptor cell layers, whereas pigmentation and lens formation were not affected. In addition, heart failure due to a weakened heartbeat and reduced circulation was noted in the pro-caspase-3 transgenic embryos. The transgenic embryos were markedly sensitive to stress conditions, such as UV irradiation at 2 or 5 mJ cm(-2). On the other hand, caspase-3 deficiency through injection of antisense morpholino oligo into embryos repressed apoptosis and enhanced stress tolerance after UV irradiation. Therefore, the caspase-3-mediated pro-apoptotic signalling pathway and its activation play critical roles in the induction of apoptosis and stress tolerance during zebrafish embryogenesis. |
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Authors:
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Michiaki Yamashita; Nanami Mizusawa; Misako Hojo; Takeshi Yabu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental biology Volume: 211 ISSN: 0022-0949 ISO Abbreviation: J. Exp. Biol. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-02 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 1874-81 Citation Subset: IM |
Affiliation:
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National Research Institute of Fisheries Science, 2-12-4 Fukuura, Yokohama 236-8648, Japan. mic@affrc.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified / embryology* Apoptosis / physiology* Caspase 3 / metabolism* DNA Primers / genetics Embryo, Nonmammalian / physiology, radiation effects Gene Expression Regulation, Developmental / genetics, physiology* Green Fluorescent Proteins In Situ Nick-End Labeling Morphogenesis / physiology* Oligonucleotides Signal Transduction / physiology* Ultraviolet Rays Zebrafish / embryology* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Oligonucleotides; 147336-22-9/Green Fluorescent Proteins; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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