Document Detail


Extension of the validation of AOAC Official Method 2005.06 for dc-GTX2,3: interlaboratory study.
MedLine Citation:
PMID:  22468349     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
AOAC Official Method(SM) 2005.06 for the determination of saxitoxin (STX)-group toxins in shellfish by LC with fluorescence detection with precolumn oxidation was previously validated and adopted First Action following a collaborative study. However, the method was not validated for all key STX-group toxins, and procedures to quantify some of them were not provided. With more STX-group toxin standards commercially available and modifications to procedures, it was possible to overcome some of these difficulties. The European Union Reference Laboratory for Marine Biotoxins conducted an interlaboratory exercise to extend AOAC Official Method 2005.06 validation for dc-GTX2,3 and to compile precision data for several STX-group toxins. This paper reports the study design and the results obtained. The performance characteristics for dc-GTX2,3 (intralaboratory and interlaboratory precision, recovery, and theoretical quantification limit) were evaluated. The mean recoveries obtained for dc-GTX2,3 were, in general, low (53.1-58.6%). The RSD for reproducibility (RSD(r)%) for dc-GTX2,3 in all samples ranged from 28.2 to 45.7%, and HorRat values ranged from 1.5 to 2.8. The article also describes a hydrolysis protocol to convert GTX6 to NEO, which has been proven to be useful for the quantification of GTX6 while the GTX6 standard is not available. The performance of the participant laboratories in the application of this method was compared with that obtained from the original collaborative study of the method. Intralaboratory and interlaboratory precision data for several STX-group toxins, including dc-NEO and GTX6, are reported here. This study can be useful for those laboratories determining STX-group toxins to fully implement AOAC Official Method 2005.06 for official paralytic shellfish poisoning control. However the overall quantitative performance obtained with the method was poor for certain toxins.
Authors:
Begoña Ben-Gigirey; María L Rodríguez-Velasco; Ana Gago-Martínez
Related Documents :
23223379 - A facile versatile polymeric monolith for multiple separations.
17120439 - Gas phase transport in gravity sewers--a methodology for determination of horizontal ga...
24752719 - When assay format matters: a case study on the evaluation of three assay formats to sup...
3739579 - A study of halothane-diethyl ether azeotrope and the oxford miniature vaporizer.
22740259 - Accurate analysis of trace earthy-musty odorants in water by headspace solid phase micr...
22553769 - Molecularly imprinted layer-coated monodisperse spherical silica microparticles toward ...
19214479 - Determination of trace elements in residual oil by high-resolution inductively coupled ...
15088739 - Determination of fuel dialkyl ethers and btex in water using headspace solid-phase micr...
1613059 - Rm values of some colchicines and colchiceinamides determined by reversed-phase thin-la...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  95     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2012 Jan-Feb
Date Detail:
Created Date:  2012-04-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  111-21     Citation Subset:  IM    
Affiliation:
European Union Reference Laboratory for Marine Biotoxins (EURLMB), CITEXVI, Campus Universitario de Vigo, Fonte das Abelleiras SN, 36310 Vigo, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparative evaluation of the association among enumeration methods and production of enterotoxins i...
Next Document:  Immunoaffinity column cleanup with liquid chromatography using postcolumn bromination for the determ...