Document Detail

Extension of the color suite available for chemical enhancement of fingerprints in blood.
MedLine Citation:
PMID:  11908604     Owner:  NLM     Status:  MEDLINE    
The use of ortho- and para-phenylenediamine (OPD & PPD respectively) for the enhancement of fingerprints in blood has been investigated. Optimal pH conditions and H2O2 concentrations have been determined using UV/Vis spectroscopy. Both OPD and PPD are effective and less hazardous alternatives to the presently used 3,3'-diaminobenzidine (DAB) for the development of blood fingerprints, especially on porous surfaces. The orange color of OPD and the purple color of PPD offer alternative colors to the brown color of DAB and the light green color of ABTS for standing out against particular backgrounds. Both OPD and PPD can be used after ninhydrin treatment, but the reverse is not the case.
Jonathan P Caldwell; Nicholas D Kim
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  47     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-22     Completed Date:  2002-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  332-40     Citation Subset:  IM    
Science and Process Engineering Division, WRONZ, Christchurch, New Zealand.
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MeSH Terms
3,3'-Diaminobenzidine / chemistry
Forensic Medicine / methods*
Hydrogen Peroxide / chemistry
Hydrogen-Ion Concentration
Indicators and Reagents / chemistry
Phenylenediamines / chemistry*
Sensitivity and Specificity
Surface Properties
Reg. No./Substance:
0/Indicators and Reagents; 0/Phenylenediamines; 106-50-3/4-phenylenediamine; 7722-84-1/Hydrogen Peroxide; 91-95-2/3,3'-Diaminobenzidine; 95-54-5/1,2-diaminobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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