Document Detail


Extension of cell life span using exogenous telomerase.
MedLine Citation:
PMID:  17634580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal human somatic cells undergo limited cell division cycles and enter irreversible replication arrest called senescence. Cellular senescence of many human cell types is regulated by the length and status of telomeric sequences, which is shortened after each round of DNA replication. Telomeres can be rejuvenated by telomerase, an enzyme which carries out de novo synthesis of telomeric DNA. Telomerase is a ribonucleoprotein complex composed minimally of telomere reverse transcriptase gene (hTERT) and RNA template (hTR), and its enzyme activity in cells is primarily limited by the level of hTERT expression. Therefore, telomerase activity in cells can be reconstituted by overexpression of hTERT, frequently resulting in extension of replicative life span or immortalization. It is well established that the effect of telomerase reconstitution on cellular life span is clearly cell type-dependent because telomere shortening is not the only limiting factor of cellular life span. However, telomerase activity appears to be a requirement for cellular immortalization, irrespective of the cell types. In this article, we discuss the detailed methods to extend the in vitro replicative life span of primary human cells by ectopic expression of hTERT.
Authors:
Mo K Kang; No-Hee Park
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  371     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-09-21     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151-65     Citation Subset:  IM    
Affiliation:
School of Dentistry, David Geffen School of Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / physiology*
Cells, Cultured
DNA Replication / physiology*
Gene Expression*
Humans
Telomerase / biosynthesis*,  genetics
Telomere / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
DE14147/DE/NIDCR NIH HHS; DE15316/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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