| Extension of cell life span using exogenous telomerase. | |
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MedLine Citation:
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PMID: 17634580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal human somatic cells undergo limited cell division cycles and enter irreversible replication arrest called senescence. Cellular senescence of many human cell types is regulated by the length and status of telomeric sequences, which is shortened after each round of DNA replication. Telomeres can be rejuvenated by telomerase, an enzyme which carries out de novo synthesis of telomeric DNA. Telomerase is a ribonucleoprotein complex composed minimally of telomere reverse transcriptase gene (hTERT) and RNA template (hTR), and its enzyme activity in cells is primarily limited by the level of hTERT expression. Therefore, telomerase activity in cells can be reconstituted by overexpression of hTERT, frequently resulting in extension of replicative life span or immortalization. It is well established that the effect of telomerase reconstitution on cellular life span is clearly cell type-dependent because telomere shortening is not the only limiting factor of cellular life span. However, telomerase activity appears to be a requirement for cellular immortalization, irrespective of the cell types. In this article, we discuss the detailed methods to extend the in vitro replicative life span of primary human cells by ectopic expression of hTERT. |
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Authors:
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Mo K Kang; No-Hee Park |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 371 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-07-19 Completed Date: 2007-09-21 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 151-65 Citation Subset: IM |
Affiliation:
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School of Dentistry, David Geffen School of Medicine, and Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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physiology* Cells, Cultured DNA Replication / physiology* Gene Expression* Humans Telomerase / biosynthesis*, genetics Telomere / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DE14147/DE/NIDCR NIH HHS; DE15316/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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