| Extension of cell life-span and telomere length in animals cloned from senescent somatic cells. | |
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MedLine Citation:
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PMID: 10784448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging. |
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Authors:
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R P Lanza; J B Cibelli; C Blackwell; V J Cristofalo; M K Francis; G M Baerlocher; J Mak; M Schertzer; E A Chavez; N Sawyer; P M Lansdorp; M D West |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 288 ISSN: 0036-8075 ISO Abbreviation: Science Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-04 Completed Date: 2000-05-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 665-9 Citation Subset: IM |
Affiliation:
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Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA. rlanza@advancedcell.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Southern Cattle / genetics* Cell Aging* Cell Division Cells, Cultured Clone Cells Cloning, Organism* DNA, Complementary Embryo Transfer Eye Proteins* Female Fibroblasts Flow Cytometry In Situ Hybridization, Fluorescence Longevity Matched-Pair Analysis Nerve Growth Factors* Nuclear Transfer Techniques* Proteins / genetics RNA, Messenger / genetics, metabolism Serpins / genetics Telomere / ultrastructure* |
| Grant Support | |
ID/Acronym/Agency:
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AG00378/AG/NIA NIH HHS; AI29524/AI/NIAID NIH HHS; GM56162/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Eye Proteins; 0/Nerve Growth Factors; 0/Proteins; 0/RNA, Messenger; 0/Serpins; 0/pigment epithelium-derived factor |
| Comments/Corrections | |
Comment In:
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Science. 2000 Apr 28;288(5466):586-7
[PMID:
10798984
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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