| Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy. | |
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MedLine Citation:
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PMID: 19243182 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods. |
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Authors:
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Alexandar L Hansen; Evgenia N Nikolova; Anette Casiano-Negroni; Hashim M Al-Hashimi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 131 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-18 Completed Date: 2009-06-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 3818-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biophysics, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Isotopes DNA / chemistry DNA Damage Kinetics Magnetic Resonance Spectroscopy / instrumentation, methods* Nucleic Acids / chemistry* RNA, Ribosomal / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Nucleic Acids; 0/RNA, Ribosomal; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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