| Extending fluorescent microsphere methods for regional organ blood flow to 13 simultaneous colors. | |
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MedLine Citation:
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PMID: 11356604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Seven fluorescent microsphere colors can be used in a single experiment to estimate regional blood flow without correcting for spillover of emitted fluorescence. To extend the method to 13 colors, we compared the accuracy of three methods for spillover correction. Fixed wavelength intensities were corrected by matrix inversion, and synchronous scan spectra were corrected by least squares fit of an overdetermined system of linear equations and by least squares fit of a sum of Gaussian and Lorentzian functions. Correction methods were validated in pigs and sheep by simultaneous injections of radioactive microspheres and fluorescent microspheres of 7, 10, and 13 different colors. We induced extreme changes in flow to create regions with low fluorescent signals bound on either side by high fluorescent signals. Blood flow was determined by radioactivity and by fluorescence using both fixed excitation and emission wavelength pairs and synchronous scanning and then corrected for spillover. Correlation between fluorescent intensity and radioactivity were excellent for all three correction methods [R2 = 0.98 +/- 0.02 (mean +/- SD)]. Low-flow regions requiring large spillover correction had systematic errors for some color combinations in all methods. We conclude that for 13 fluorescent colors spillover error can be minimized so that all three correction methods provide accurate estimates of regional blood flow. |
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Authors:
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C Schimmel; D Frazer; R W Glenny |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 280 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-17 Completed Date: 2001-06-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H2496-506 Citation Subset: IM |
Affiliation:
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Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle, Washington 98195, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Flow Velocity / physiology Color / standards* Fluorescent Dyes / analysis*, metabolism Microspheres Models, Cardiovascular* Normal Distribution Predictive Value of Tests Radioactive Tracers Regional Blood Flow / physiology* Regression Analysis Reproducibility of Results Sheep Spectrometry, Fluorescence / instrumentation, methods* Swine |
| Grant Support | |
ID/Acronym/Agency:
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P01-HL-24163/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Radioactive Tracers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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