| The extended cell panel assay characterizes the relationship of prion strains RML, 79A, and 139A and reveals conversion of 139A to 79A-like prions in cell culture. | |
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MedLine Citation:
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PMID: 22379091 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three commonly used isolates of murine prions, 79A, 139A, and RML, were derived from the so-called Chandler isolate, which was obtained by propagating prions from scrapie-infected goat brain in mice. RML is widely believed to be identical with 139A; however, using the extended cell panel assay (ECPA), we here show that 139A and RML isolates are distinct, while 79A and RML could not be distinguished. We undertook to clone 79A and 139A prions by endpoint dilution in murine neuroblastoma-derived PK1 cells. Cloned 79A prions, when returned to mouse brain, were unchanged and indistinguishable from RML by ECPA. However, 139A-derived clones, when returned to brain, yielded prions distinct from 139A and similar to 79A and RML. Thus, when 139A prions were transferred to PK1 cells, 79A/RML-like prions, either present as a minor component in the brain 139A population or generated by mutation in the cells, were selected and, after being returned to brain, were the major if not only component of the population. |
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Authors:
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Anja M Oelschlegel; Mohammad Fallahi; Shannon Ortiz-Umpierre; Charles Weissmann |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-02-29 |
Journal Detail:
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Title: Journal of virology Volume: 86 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-04-11 Completed Date: 2012-06-18 Revised Date: 2013-02-21 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 5297-303 Citation Subset: IM |
Affiliation:
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Department of Infectology, Scripps Florida, Jupiter, Florida, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Cell Line Cells, Cultured Mice Mice, Inbred C57BL Prions / isolation & purification*, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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1R01NS067214/NS/NINDS NIH HHS; 1R01NSO59543//PHS HHS; R01 NS067214/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Prions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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