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The extended cell panel assay characterizes the relationship of prion strains RML, 79A, and 139A and reveals conversion of 139A to 79A-like prions in cell culture.
MedLine Citation:
PMID:  22379091     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three commonly used isolates of murine prions, 79A, 139A, and RML, were derived from the so-called Chandler isolate, which was obtained by propagating prions from scrapie-infected goat brain in mice. RML is widely believed to be identical with 139A; however, using the extended cell panel assay (ECPA), we here show that 139A and RML isolates are distinct, while 79A and RML could not be distinguished. We undertook to clone 79A and 139A prions by endpoint dilution in murine neuroblastoma-derived PK1 cells. Cloned 79A prions, when returned to mouse brain, were unchanged and indistinguishable from RML by ECPA. However, 139A-derived clones, when returned to brain, yielded prions distinct from 139A and similar to 79A and RML. Thus, when 139A prions were transferred to PK1 cells, 79A/RML-like prions, either present as a minor component in the brain 139A population or generated by mutation in the cells, were selected and, after being returned to brain, were the major if not only component of the population.
Authors:
Anja M Oelschlegel; Mohammad Fallahi; Shannon Ortiz-Umpierre; Charles Weissmann
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-02-29
Journal Detail:
Title:  Journal of virology     Volume:  86     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-11     Completed Date:  2012-06-18     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5297-303     Citation Subset:  IM    
Affiliation:
Department of Infectology, Scripps Florida, Jupiter, Florida, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism
Cell Line
Cells, Cultured
Mice
Mice, Inbred C57BL
Prions / isolation & purification*,  physiology*
Grant Support
ID/Acronym/Agency:
1R01NS067214/NS/NINDS NIH HHS; 1R01NSO59543//PHS HHS; R01 NS067214/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Prions
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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