Document Detail

Expressions of p-c-jun and CyclinD1 between Vascular Smooth Muscle Cells and Endothelial Cells Exposured to Cigarette Smoke Extract.
MedLine Citation:
PMID:  23643008     Owner:  NLM     Status:  In-Data-Review    
Objective To investigate the cell viabilities of vascular smooth muscle cells and vascular endothelial cells stimulated by cigarette smoke extract(CSE) . Methods The CSE was prepared by smoke-bubbled phosphate buffered saline(PBS) generation.After culturing cells with different concentrations of CSE, we used the cell counting kit-8 to determine the cell viability.The expression levels of c-jun and cyclinD1 were analyzed through Western blot.The c-jun plasmid was transfected to detect the change of cyclinD1 expression. Results The smooth muscle cell viability increased when the CSE concentration ranged 0.625%-10%, whereas the endothelial cells viability decreased when exposed to the CSE concentration. After exposure to CSE for 48 hours, there was no difference in c-jun expression between toxin group and PBS group;however, the expression of p-c-jun in the smooth muscle cells significantly increased in the toxin groups than in the PBS group(P<0.05) and the expression of p-c-jun in the vascular endothelial cells significantly decreased(P<0.05) . The level of cyclinD1 significantly increased after exposed to CSE, and its expression level also increased in respond to the c-jun overexpression. Conclusion CSE can enhance the proliferation of vascular smooth muscle cells and decrease in the activity of endothelial cells proliferation, which may be explained by the phosphorylation of c-jun and the expression of cyclinD1.
Li Tian-Jia; Liu Chang-Wei; Huang Ze-Bin; Ni Leng; Lai Zhi-Chao; Wu Li-Fei; Liu Bao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae     Volume:  35     ISSN:  1000-503X     ISO Abbreviation:  Zhongguo Yi Xue Ke Xue Yuan Xue Bao     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006230     Medline TA:  Zhongguo Yi Xue Ke Xue Yuan Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
Department of Vascular Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100032, China.
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