Document Detail

Expression of voltage-gated calcium channel α(2)δ(4) subunits in the mouse and rat retina.
MedLine Citation:
PMID:  23296739     Owner:  NLM     Status:  MEDLINE    
High-voltage activated Ca channels participate in multiple cellular functions, including transmitter release, excitation, and gene transcription. Ca channels are heteromeric proteins consisting of a pore-forming α(1) subunit and auxiliary α(2)δ and β subunits. Although there are reports of α(2)δ(4) subunit mRNA in the mouse retina and localization of the α(2)δ(4) subunit immunoreactivity to salamander photoreceptor terminals, there is a limited overall understanding of its expression and localization in the retina. α(2)δ(4) subunit expression and distribution in the mouse and rat retina were evaluated by using reverse transcriptase polymerase chain reaction, western blot, and immunohistochemistry with specific primers and a well-characterized antibody to the α(2)δ(4) subunit. α(2)δ(4) subunit mRNA and protein are present in mouse and rat retina, brain, and liver homogenates. Immunostaining for the α(2)δ(4) subunit is mainly localized to Müller cell processes and endfeet, photoreceptor terminals, and photoreceptor outer segments. This subunit is also expressed in a few displaced ganglion cells and bipolar cell dendrites. These findings suggest that the α(2)δ(4) subunit participates in the modulation of L-type Ca(2+) current regulating neurotransmitter release from photoreceptor terminals and Ca(2+)-dependent signaling pathways in bipolar and Müller cells.
Luis Pérez De Sevilla Müller; Janelle Liu; Alexander Solomon; Allen Rodriguez; Nicholas C Brecha
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  521     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-06-05     Completed Date:  2014-01-13     Revised Date:  2014-08-05    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2486-501     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Blotting, Western
Brain Chemistry / physiology
Calcium Channels / biosynthesis*
Dendrites / metabolism
Image Processing, Computer-Assisted
Liver / metabolism
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Confocal
Microscopy, Fluorescence
Photoreceptor Cells, Vertebrate / physiology
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Retina / cytology,  metabolism*
Grant Support
Reg. No./Substance:
0/Cacna2d2 protein, mouse; 0/Calcium Channels

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