Document Detail

Expression of vasodilator-stimulated phosphoprotein in human placenta: possible implications in trophoblast invasion.
MedLine Citation:
PMID:  11756574     Owner:  NLM     Status:  MEDLINE    
The vasodilator-stimulated phosphoprotein (VASP) is a 46 kDa protein present at the leading edge of migrating cells. Because trophoblastic cell migration and invasion are critical stages for the achievement of successful implantation and development of the placenta, we investigated VASP expression in different cell types of the human placenta throughout pregnancy by immunohistochemistry and Western blot analysis. We also studied the effect of leukaemia inhibitory factor (LIF) and transforming growth factor-beta1 (TGF-beta1) on the regulation of VASP expression in first trimester placental tissue explants. We found that VASP is expressed throughout pregnancy by a variety of cells in the human placenta. The strongest VASP immunoreactivity was observed in the first trimester. In these samples, the most intense immunoreactivity was in invasive trophoblasts, namely, extravillous cells of the anchoring villi, distal extravillous trophoblasts of cell columns, and also in cells of placental fibrinoids. We also found that LIF (but not TGF-beta1) has a stimulatory effect on VASP expression in placental explants. The strong VASP immunoreactivity in invasive trophoblasts suggests that this protein may be associated with trophoblastic cell motility and may have a role in implantation and trophoblastic cell invasion. We speculate that one of the effects of LIF in successful pregnancy may be its induction of VASP expression.
Umit A Kayisli; Belgin Selam; Ramazan Demir; Aydin Arici
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  8     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  88-94     Citation Subset:  IM    
Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Yale University School of Medicine,333 Cedar Street, New Haven, CT 06520-8063, USA.
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MeSH Terms
Blood Proteins / metabolism
Cell Adhesion Molecules / genetics,  metabolism*
Cell Movement / physiology*
Cells, Cultured
Culture Media, Serum-Free
Growth Inhibitors / pharmacology
Leukemia Inhibitory Factor
Lymphokines / pharmacology
Microfilament Proteins / metabolism
Phosphoproteins / genetics,  metabolism*
Placenta / cytology,  drug effects,  metabolism*
Pregnancy Trimesters
Transforming Growth Factor beta / pharmacology
Transforming Growth Factor beta1
Trophoblasts / metabolism*
Reg. No./Substance:
0/Blood Proteins; 0/Cell Adhesion Molecules; 0/Culture Media, Serum-Free; 0/Growth Inhibitors; 0/Interleukin-6; 0/LIF protein, human; 0/Leukemia Inhibitory Factor; 0/Lymphokines; 0/Microfilament Proteins; 0/Phosphoproteins; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/vasodilator-stimulated phosphoprotein

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