| Expression of vascular endothelial growth factor and its receptor Flk-1 in human neuroblastoma using in situ hybridization. | |
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MedLine Citation:
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PMID: 12483647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Although angiogenic factors may play an important role in the biology of neuroblastoma, which frequently spreads hematogenously, the mechanism remains unclear. The authors studied tumor progression and invasion from the perspective of angiogenesis and sought to understand the features of this type of tumor. METHODS: Thirty-one specimens were resected from patients with neuroblastoma and the expression of vascular endothelial growth factor (VEGF), and its receptor (Flk-1) was examined using immunohistochemistry. The authors looked for correlations among the expressions of VEGF and its receptor with various clinicopathologic factors. In addition, they examined the expression and location of VEGF and Flk-1 mRNA in 10 primary neuroblastoma using in situ hybridization. RESULTS: Both in situ hybridization and immunohistochemistry showed the presence of VEGF expression within the neuroblastoma cells. We found VEGF mRNA in neuroblastoma cells but not vascular endothelial cells according to in situ hybridization. Further, Flk-1 mRNA was present both in neuroblastoma cells and vascular endothelial cells. The level of VEGF expression was higher in unfavorable histology, using the criteria of Shimada, than in favorable histology. CONCLUSION: The authors suggest that paracrine and autocrine systems are involved in the angiogenesis of neuroblastoma, and the expression of VEGF correlates with the prognosis in neuroblastoma. |
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Authors:
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Masahiro Fukuzawa; Hiroaki Sugiura; Tsugumichi Koshinaga; Taro Ikeda; Noritugu Hagiwara; Tatsuo Sawada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 37 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-16 Completed Date: 2003-02-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1747-50 Citation Subset: IM |
Copyright Information:
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Copyright 2002, Elsevier Science (USA). All rights reserved. |
Affiliation:
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First Department of Surgery, Nihon University School of Medicine, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Disease Progression Endothelial Growth Factors / analysis*, genetics Female Humans Immunohistochemistry In Situ Hybridization / methods* Infant Infant, Newborn Intercellular Signaling Peptides and Proteins / analysis*, genetics Lymphokines / analysis*, genetics Male Neoplasm Recurrence, Local / chemistry, pathology Neoplasm Staging Neovascularization, Pathologic / pathology Neuroblastoma / chemistry*, pathology Prognosis RNA, Messenger / analysis Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 / analysis* Vascular Endothelial Growth Factors |
| Chemical | |
Reg. No./Substance:
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0/Endothelial Growth Factors; 0/Intercellular Signaling Peptides and Proteins; 0/Lymphokines; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2 |
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