Document Detail


Expression of the urokinase plasminogen activator receptor is transiently required during "priming" of PC12 cells in nerve growth factor-directed cellular differentiation.
MedLine Citation:
PMID:  11170184     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously identified the urokinase plasminogen activator receptor (UPAR) as a gene induced by nerve growth factor (NGF), but not by epidermal growth factor (EGF), in PC12 cells (Farias-Eisner et al. [2000] J. Neurosci. 20:230-239). Antisense oligonucleotides for the UPAR mRNA or an antibody directed against UPAR protein, added simultaneously with NGF, block NGF-induced morphological and biochemical differentiation of PC12 cells. In this report, we show that anti-UPAR antibody blocks morphological differentiation and the expression of two NGF-specific secondary response genes, collagenase-1 and transin, in PC12 cells only during the first 2 hr following NGF exposure. These data suggest that induced UPAR expression is required only over a short period of time following exposure to NGF for the differentiation program in PC12 cells to proceed. For two models of "primed" PC12 cells, we found that UPAR expression and function are not required for NGF-induced differentiation. UPAR and the secondary response genes collagenase-1 and transin are not induced in "primed" PC12 cells in response to NGF, and anti-UPAR antibody does not block morphological differentiation in these cells. Our data suggests that UPAR is required only transiently during the "priming" of PC12 cells in NGF-induced PC12 cell differentiation.
Authors:
R Farias-Eisner; L Vician; S Reddy; R Basconcillo; S A Rabbani; Y Y Wu; R A Bradshaw; H R Herschman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  63     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-04-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Department of Obstetrics and Gynecology, UCLA Center for the Health Sciences, Los Angeles, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / pharmacology
Cell Differentiation / drug effects
Collagenases / genetics
Gene Expression / drug effects,  physiology
Matrix Metalloproteinase 3 / genetics
Nerve Growth Factor / pharmacology*
Neurons / cytology*,  physiology*
Neutralization Tests
PC12 Cells
Rats
Receptors, Cell Surface / genetics*,  immunology
Receptors, Urokinase Plasminogen Activator
Grant Support
ID/Acronym/Agency:
AG 09735/AG/NIA NIH HHS; K12HD01281-01/HD/NICHD NIH HHS; NS28660/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Plaur protein, rat; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; 9061-61-4/Nerve Growth Factor; EC 3.4.24.-/Collagenases; EC 3.4.24.17/Matrix Metalloproteinase 3

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