Document Detail


Expression of urokinase plasminogen activator and the urokinase plasminogen activator receptor in myeloma cells.
MedLine Citation:
PMID:  10929035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Binding of urokinase (uPA) to its receptor (uPAR; CD87) focuses proteolytic activity on the cell surface and this system is of importance in malignant matrix degradation and tumour invasion. By immunocytochemistry and flow cytometry, we found that primary myeloma cells and myeloma cell lines expressed uPA and uPAR. Soluble uPA was present in cell line supernatants and lysates in low concentrations. In cell lines, uPA and uPAR were located both on the cell surface and intracellularly, but the expression of both proteins was low. Higher levels of uPAR was detected on the cell surface of primary myeloma cells. When primary myeloma cells were gated by CD45 expression, stronger expression was found on immature CD45+ cells than on mature CD45-/dim cells. Finally, both myeloma cell lines and primary cells were able to cleave a uPA-specific substrate showing that the uPA system is functionally active. We conclude that myeloma cells are able to produce uPA and uPAR. This opens up a possible role of the uPA system in myeloma cell invasion and in the proteolytic digestion of bone matrix.
Authors:
O Hjertner; G Qvigstad; H Hjorth-Hansen; C Seidel; J Woodliff; J Epstein; A Waage; A Sundan; M Börset
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  109     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-31     Completed Date:  2000-08-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  815-22     Citation Subset:  IM    
Affiliation:
The Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, Trondheim, Norway. oyvind.hjertner@medisin.ntnu.no
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MeSH Terms
Descriptor/Qualifier:
Flow Cytometry
Humans
Immunohistochemistry
Multiple Myeloma / metabolism*
Plasminogen Activators / analysis*
Receptors, Cell Surface / analysis*
Receptors, Urokinase Plasminogen Activator
Tumor Cells, Cultured
Urokinase-Type Plasminogen Activator / analysis*
Chemical
Reg. No./Substance:
0/PLAUR protein, human; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

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