Document Detail


Expression of types I and IV collagen genes in normal and in modulated corneal endothelial cells.
MedLine Citation:
PMID:  2914755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of type I and type IV collagen genes was studied in normal endothelial cells and in modulated endothelial cells (formerly designated as fibroblastic corneal endothelial cells: FCEC). There appear to be distinct collagen phenotypes in these cells; normal cells produce type IV collagen as a major type, while the modulated cells produce predominantly type I collagen. The absence of type I synthesis in normal cells, and absence of type IV collagen in FCEC were confirmed by immunoblot analysis of the accumulated collagen in the cellular and medium layers in cultures of these cells. When collagen mRNAs were translated in a cell-free system, the results indicate that neither type I collagen RNAs in the normal cells, nor type IV collagen RNAs in the modulated cells were translated. Using cloned cDNA probes, the relative quantities of transcripts of these collagens were determined by slot blot hybridization; normal cells, which synthesize no detectable type I collagen, were found to contain type I collagen RNAs in similar amounts as did the modulated cells, while the levels of type IV RNAs in modulated cells were as high as those in normal cells, in spite of the absence of their translational products. Northern blot analysis demonstrated that there are no distinct differences in the size of type I collagen RNAs in the normal and modulated cells. In contrast, type IV collagen RNA in the modulated cells was much smaller than the normal cells, suggesting that the alpha 1(IV) RNA is readily degraded.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
E P Kay
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  30     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-03-20     Completed Date:  1989-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  260-8     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
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MeSH Terms
Descriptor/Qualifier:
Animals
Collagen / classification,  genetics*
Endothelium, Corneal / physiology*
Gene Expression Regulation*
Genes*
Protein Biosynthesis
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
EY-06431/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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