| Expression of two mRNAs for immunoglobulin lambda chains in the rat. | |
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MedLine Citation:
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PMID: 2447492 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have analyzed the expression of immunoglobulin lambda chains in the rat by hybridizing RNA from various sources with C lambda 1-like and C lambda 2-like sequences recovered from a rat genomic library. A 1.0 kb lambda 2-like sequence is readily detected in lambda-producing hybridomas and in normal rat spleen RNAs; a 1.0 kb lambda 1-like message is also present, although at much lower levels. An additional 700 b.p. C lambda 2-like fragment is found in all normal rat spleens, and presumably represents a defective message. The nucleotide sequence of one cDNA clone isolated from the lambda-producing hybridoma G36/1 shows a lambda 2-like sequence, and six lambda-secreting hybridomas produced from the spleen of a kappa-suppressed rat all express a C lambda 2-like message. The great majority of rat lambda chains therefore appear to be lambda 2-like. Northern blot analysis of RNA from the spleen of this kappa-suppressed rat shows a considerable increase in the expression of both lambda 2-like and (at lower levels) lambda 1-like message. The coordinate rise of lambda 1 and lambda 2 RNA in this rat suggests that there may be at least two functional lambda chain genes in the rat, although there is as yet no evidence for the existence of rat lambda 1-like proteins. |
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Authors:
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D F Zhou; K Z Guo; S A Tilles; G A Gutman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular immunology Volume: 24 ISSN: 0161-5890 ISO Abbreviation: Mol. Immunol. Publication Date: 1987 Nov |
Date Detail:
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Created Date: 1988-01-25 Completed Date: 1988-01-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1151-8 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line Electrophoresis, Agar Gel Hybridomas / immunology Immunoglobulin lambda-Chains / biosynthesis, genetics* Molecular Sequence Data RNA / analysis RNA, Messenger / analysis* Rats Rats, Inbred Strains Spleen / analysis |
| Grant Support | |
ID/Acronym/Agency:
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AI-14774/AI/NIAID NIH HHS; AI-21366/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin lambda-Chains; 0/RNA, Messenger; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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