Document Detail


Expression of tuberalin II, alpha-subunit of glycoprotein hormones and beta-thyrotropin hormone in the pars tuberalis of the rat: immunocytochemical evidence for pars tuberalis-specific cell types.
MedLine Citation:
PMID:  19829005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increasing evidence suggests that the hypophyseal pars tuberalis (PT) plays a key role in the transduction of light/dark (melatonin) information to the endocrine system. It has been shown that PT-specific cells express melatonin receptors and thyrotropin hormone (TSH) subunits. However, these cells do not resemble thyrotrophs or any other of the pars distalis (PD) cells. There is evidence that PT-specific cells secrete a glycoprotein hormone designated as 'tuberalin'. We have identified a putative tuberalin of 21 kDa (tuberalin II) and have raised antibodies against it. To further investigate whether tuberalin II is a distinct secretory compound of the PT, absorption studies of antituberalin II with TSH or with an extract of the rat PD containing beta-TSH, beta-luteinizing hormone (LH) and the common alpha-subunit of glycoprotein hormones (GSU), were performed. Neither of the absorption tests abolished the immunoreactivity of the PT to antituberalin II, suggesting that tuberalin II is different from TSH or the other PD glycoprotein hormones. Double immunofluorescence analyses using antibodies against tuberalin II, beta-TSH and GSU revealed that in the developing and adult PT there are 3 populations of PT-specific cells expressing tuberalin II and GSU (type 1), beta-TSH and GSU (type 2) and tuberalin II, beta-TSH and GSU (type 3). This further indicates that tuberalin II and beta-TSH correspond to different compounds and that they may be expressed either by different cells types or coexpressed in a 3rd cell type. The distribution and temporal expression of tuberalin II, beta-TSH, beta-LH and GSU were investigated in the developing pituitary gland. At E14.5, tuberalin II and GSU were expressed by cells of the PT primordium but not by the PD and pars intermedia primordia. The onset of expression of beta-TSH, beta-LH and GSU in cells of the PD occurred about 1 day later, further indicating the distinct nature of tuberalin II and supporting the earlier view that the secretion of polypeptides from the fetal rat pituitary gland begins in PT-specific cells.
Authors:
Mar??a Montserrat Guerra; Esteban M Rodr??guez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-15
Journal Detail:
Title:  Neuroendocrinology     Volume:  90     ISSN:  1423-0194     ISO Abbreviation:  Neuroendocrinology     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-15     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0035665     Medline TA:  Neuroendocrinology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  269-82     Citation Subset:  IM    
Affiliation:
Instituto de Anatom??a, Histolog??a y Patolog??a, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. monserratguerra@uach.cl
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count
Female
Fluorescent Antibody Technique
Glycoprotein Hormones, alpha Subunit / metabolism*
Glycoproteins / metabolism*
Immunohistochemistry
Luteinizing Hormone, beta Subunit / metabolism
Male
Pituitary Gland / embryology,  growth & development*,  metabolism*
Rats
Rats, Sprague-Dawley
Thyrotropin, beta Subunit / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Glycoprotein Hormones, alpha Subunit; 0/Glycoproteins; 0/Luteinizing Hormone, beta Subunit; 0/Thyrotropin, beta Subunit; 0/tuberalin II protein, rat

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