Document Detail


Expression and translation of the COX-1b gene in human cells - no evidence for generation of COX-1b protein.
MedLine Citation:
PMID:  23362192     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Cyclooxygenase 1b (COX-1b) is a splice variant of COX-1, containing a retained intron 1 within the signal peptide sequence. COX-1b mRNA is found in many species, but the existence of a functionally active protein is controversially discussed, possibly related to different species-dependent lengths of intron 1. The human intron 1 comprises 94 bp and the resulting frame shift at the intron 1-exon 2 junction creates a premature stop codon. Nevertheless, full length human COX-1b protein expression, including translated intron 1 and the signal peptide, has been reported and was explained by frame shift repair. In this study the fate of COX-1b mRNA in a human overexpression system is analysed. Independent of the hypothetical frame shift repair mechanism, splicing of COX-1b intron 1, resulting in COX-1 mRNA and removal of the signal peptide during protein maturation, with subsequent generation of a COX-1 protein is demonstrated.
Authors:
Christina Reinauer; Petra Censarek; Gernot Kaber; Artur-Aron Weber; Gerhard Steger; Thorsten Klamp; Karsten Schrör
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-29
Journal Detail:
Title:  Biological chemistry     Volume:  -     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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