Document Detail


Expression of transient receptor potential (TRP) channels in human and murine osteoblast-like cells.
MedLine Citation:
PMID:  19115145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preservation of bone mass relies on adequate proliferation, differentiation, secretion of matrix proteins and rate of apoptosis of the bone-forming osteoblasts. Although growing body of evidence indicates that the transient receptor potential (TRP) channels play important roles in numerous cellular functions, limited information is available about the TRP channels in osteoblasts. Here, we inventoried the gene expression and addressed some roles of the TRP channels in various osteoblast-like cells. The transcripts of canonical TRP (TRPC) channels were revealed for TRPC1, TRPC3, TRPC4 and TRPC6 in human MG-63, SaOS and U2 OS osteoblasts while transcripts for TRPC2, TRPC4, TRPC6 and TRPC7 were observed in the murine MC3T3 osteoblasts. PCR products were shown for the melastatin-related TRP (TRPM) channels TRPM4, TRPM6, TRPM7 and TRPM8 in all cell lines. The TRPM1 was specifically expressed by murine MC3T3 cells while the TRPM3 transcripts were revealed solely in human osteoblast-like cells. Transcripts for TRPV2 and TRPV4 were shown in osteoblastic cells. By interfering RNA approaches, the TRPC1 channels in osteoblasts were shown to be responsible for the capacitative calcium entry (CCE) and for the stimulation of cell proliferation by platelet-derived growth factor. On the other hand, interfering RNA-mediated abrogation of the expression of TRPM7, known as calcium and magnesium channels, resulted in the reduction of both basal and growth factor-stimulated osteoblastic cell proliferation. Our results provide the first complete reference for the gene expression of TRP channels in osteoblasts and point to their importance in cell proliferation.
Authors:
Elie Abed; Dominique Labelle; Corine Martineau; Andrew Loghin; Robert Moreau
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-27
Journal Detail:
Title:  Molecular membrane biology     Volume:  26     ISSN:  1464-5203     ISO Abbreviation:  Mol. Membr. Biol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-02-27     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430797     Medline TA:  Mol Membr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  146-58     Citation Subset:  IM    
Affiliation:
Laboratoire du Métabolisme osseux, BioMed, Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Gene Expression
Humans
Mice
Osteoblasts / cytology*
RNA, Messenger / analysis
TRPM Cation Channels / analysis,  genetics
TRPV Cation Channels / analysis,  genetics
Transient Receptor Potential Channels / analysis*,  genetics
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/TRPM Cation Channels; 0/TRPV Cation Channels; 0/Transient Receptor Potential Channels

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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