Document Detail


Expression of transduced carcinoembryonic antigen gene in human rhabdomyosarcoma inhibits metastasis.
MedLine Citation:
PMID:  8813148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carcinoembryonic antigen (CEA) is a highly glycosylated cell surface glycoprotein belonging to the immunoglobulin superfamily. CEA has been involved in vitro in adhesion mechanisms, but little is known about the function of this glycoprotein in vivo in normal tissue differentiation and malignancy. With regard to the relationship between CEA expression and tissue differentiation, it has been reported that transfection of the CEA gene in rat L6 myoblasts results in a complete block of myogenic differentiation. To extend investigations to the transformed myogenic counterpart and examine CEA effects on differentiation and malignancy outside the colon system, we have transfected the human CEA gene in human rhabdomyosarcoma cells. Human rhabdomyosarcoma cells transfected with the CEA gene correctly expressed membrane CEA anchored via glycosylphosphatidylinositol and secreted CEA in the medium. CEA gene transfer in human rhabdomyosarcoma cells, which display a limited differentiation ability, does not further inhibit myogenic differentiation or alter in vitro proliferation or natural killer sensitivity. CEA transfection does not affect s.c. growth in nude mice, but the ectopic expression of CEA in human rhabdomyosarcoma cells can strongly inhibit their metastatic ability to lungs and adrenals after i.v. injection. The impairment of metastatic potential correlates with a reduction in the homotypic adhesion properties of the cells. These data suggest that CEA, in some systems, can interfere with intercellular adhesion and, at least for cells not metastatic to the liver, can act as an anti-metastatic molecule.
Authors:
L Landuzzi; F Frabetti; I Rossi; C Griffoni; C De Giovanni; G Nicoletti; P Nanni; R Miniero; G Palmieri; A Santoni; P L Lollini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  56     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-21     Completed Date:  1996-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4503-8     Citation Subset:  IM    
Affiliation:
Cancer Research Institute, University of Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Gland Neoplasms / genetics,  secondary
Animals
Carcinoembryonic Antigen / biosynthesis,  genetics,  physiology*
Cell Differentiation
DNA, Complementary / genetics
Female
Gene Expression Regulation, Neoplastic*
Glycosylphosphatidylinositols / metabolism
Humans
Killer Cells, Natural / immunology
Lung Neoplasms / genetics,  secondary
Mice
Mice, Nude
Muscles / pathology
Neoplasm Metastasis / genetics*
Recombinant Fusion Proteins / biosynthesis,  metabolism
Rhabdomyosarcoma / genetics*,  pathology,  secondary
Soft Tissue Neoplasms / genetics*,  pathology
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Carcinoembryonic Antigen; 0/DNA, Complementary; 0/Glycosylphosphatidylinositols; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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