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Expression of tissue transglutaminase on primary olfactory ensheathing cells cultures exposed to stress conditions.
MedLine Citation:
PMID:  22222252     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tissue transglutaminase (TG2), a multifunctional enzyme implicated in cellular proliferation and differentiation processes, plays a modulatory role in the cell response to stressors. Herein, we used olfactory ensheathing cells (OECs), representing an unusual population of glial cells to promote axonal regeneration and to provide trophic support, as well as to assess whether the effect of some Growth Factors (GFs), NGF, bFGF or GDNF, on TG2 overexpression induced by stress conditions, such as glutamate or lipopolysaccaride (LPS). Glial Fibrillary Acidic Protein (GFAP) and vimentin were used as markers of astroglial differentiation and cytoskeleton component, respectively. Glutamate or LPS treatment induced a particular increase of TG2 expression. A pre-treatment of the cells with the GFs restored the levels of the protein to that of untreated ones. Our results demonstrate that the treatment of OECs with the GFs was able to restore the OECs oxidative status as modified by stress, also counteracting TG2 overexpression. It suggests that, in OECs, TG2 modulation or inhibition induced by GFs might represent a therapeutic target to control the excitotoxicity and/or inflammation, which are involved in several acute and chronic brain diseases.
Authors:
Agata Campisi; Michela Spatuzza; Antonella Russo; Giuseppina Raciti; Angelo Vanella; Stefania Stanzani; Rosalia Pellitteri
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-24
Journal Detail:
Title:  Neuroscience research     Volume:  -     ISSN:  1872-8111     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ireland Ltd.
Affiliation:
Department of Drug Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
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