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Expression of thymic stromal lymphopoietin in canine atopic dermatitis.
MedLine Citation:
PMID:  23331680     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background -  In humans, thymic stromal lymphopoietin (TSLP) plays a central role in the development of allergic inflammation, such as atopic dermatitis (AD), but it is unknown whether it is involved in the pathogenesis of canine AD (CAD). Hypothesis/Objectives -  Our aim was to characterize canine TSLP and to assess its expression in CAD. Methods -  Canine TSLP was identified based on sequence homology with human TSLP and the complementary DNA (cDNA) cloned by RT-PCR. Real-time quantitative RT-PCR was established to assess the expression of canine TSLP in cultured canine keratinocytes and in skin biopsy specimens from lesional and nonlesional skin of 12 dogs with CAD and eight healthy control dogs. Results -  Partial canine TSLP cDNA was cloned and characterized. It contained four exons that shared 70 and 73% nucleotide identity with human and equine TSLP, respectively, encoding the signal peptide and full-length secreted protein. We found significantly increased TSLP expression in lesional and nonlesional skin of dogs with CAD compared with healthy control dogs (P < 0.05), whereas no difference was measured between lesional and nonlesional samples. In cultured primary canine keratinocytes, we found increased TSLP expression after stimulation with house dust mite allergen extract or Toll-like receptor ligands lipopolysaccharide and poly I:C. Conclusions and clinical importance -  Increased TSLP expression in the skin of dogs with CAD supports an involvement of TSLP in the pathogenesis of CAD similar to that in humans. Further studies should elucidate the function and therapeutic potential of TSLP in CAD.
Authors:
Jolanta Klukowska-Rötzler; Ludovic Chervet; Eliane J Müller; Petra Roosje; Eliane Marti; Jozef Janda
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Veterinary dermatology     Volume:  24     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  54-e14     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD.
Affiliation:
Department of Clinical Research-VPH, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, CH-3012 Bern, Switzerland Molecular Dermatology, Institute for Animal Pathology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland Division of Clinical Dermatology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Länggassstrasse 128, CH-3012 Bern, Switzerland DermFocus, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland.
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