Document Detail


Expression of thermotolerance following microinjection of glutathione disulfide.
MedLine Citation:
PMID:  2455919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asynchronous Chinese hamster ovary cells were microinjected with glutathione disulfide (GSSG). Successfully injected cells were scored by coinjecting FITC-dextran with GSSG, followed by fluorescent microscopy. After microinjection, cells were incubated for 2.5 h at 37 degrees C to permit thermotolerance development and then heated at 45 degrees C for 40 min. Cellular heat sensitivity was quantitated by counting the number of grains per cell after labeling heated cells with tritiated amino acids and processing for autoradiography. The data show that microinjection of GSSG induced thermotolerance which increased the number of grains per cell up to 500% of controls. Cells that were exposed to similar concentrations of GSSG in culture medium without microinjection or microinjected without GSSG did not develop thermotolerance.
Authors:
C K Lumpkin; K J Henle; G Sammartino; G T Nolen; J M Taylor
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  115     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-18     Completed Date:  1988-08-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  202-10     Citation Subset:  IM; S    
Affiliation:
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Cell Line / drug effects
Cell Survival
Dextrans
Fluorescein-5-isothiocyanate
Fluoresceins
Glutathione / analogs & derivatives*,  pharmacology
Glutathione Disulfide
Hot Temperature*
Microinjections
Microscopy, Fluorescence
Phenotype
Thiocyanates
Grant Support
ID/Acronym/Agency:
CA 33405/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Thiocyanates; 27025-41-8/Glutathione Disulfide; 3326-32-7/Fluorescein-5-isothiocyanate; 70-18-8/Glutathione; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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