| Expression of testicular angiotensin-converting enzyme in adult spontaneously hypertensive rats. | |
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MedLine Citation:
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PMID: 19419949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies demonstrated that one isoform of angiotensin-converting enzyme named testicular or germinal (tACE) is localized in postmeiotic male germ cells and is essential for fertilizing ability of spermatozoa. Hypertension in spontaneously hypertensive rats (SHR) is androgen-dependent and reduction in male gametes is reported in this experimental conditions. Expression of tACE was not studied under conditions of spontaneous hypertension. The aim of this work is to characterize immuno-expression of tACE in the testis of adult (16-week-old) SHR rats in relation to the changes in blood pressure and serum testosterone level. In 82% of adult SHR, the immuno-expression of tACE followed the normal stage-specific pattern. Destructive testicular changes, germ cells depletion have been observed in 18% of 16-week-old SHR and stronger expression of tACE in stages 8-11 compared to controls was detected. As a result stage specificity in SHR was not as evident as in control. No reaction was found in germ cell depleted tubules in which elongated spermatids were absent. Degenerating germ cells exhibited strong immunostaining comparable to that in residual bodies. The blood pressure was significantly higher in SHR and testosterone levels were more than twice but non-significantly elevated. There was no clear correlation between testicular structural changes, blood pressure level values or serum testosterone levels. Expression of tACE in postmeiotic germ cells, specifically altered by SHR, suggested possible involvement of components of renin-angiotensin system in the process of spermiogenesis. Loss of enzyme expression we found in germ cell depleted tubules in SHR is due to absence of corresponding stages of spermatid differentiation. Therefore, tACE can be used as a marker for germ cell depletion due to hypertension and other pathological conditions. |
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Authors:
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Nina Atanassova; Emilia Lakova; Yordanka Bratchkova; Genka Krasteva; Miroslav Donchev |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society Volume: 47 ISSN: 1897-5631 ISO Abbreviation: Folia Histochem. Cytobiol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-05-07 Completed Date: 2009-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502651 Medline TA: Folia Histochem Cytobiol Country: Poland |
Other Details:
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Languages: eng Pagination: 117-22 Citation Subset: IM |
Affiliation:
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Institute of Experimental Morphology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure Body Weight Hypertension / blood, enzymology*, physiopathology Male Organ Size Peptidyl-Dipeptidase A / metabolism* Rats Rats, Inbred SHR Rats, Wistar Seminiferous Epithelium / enzymology Spermatids / enzymology, pathology Testis / enzymology*, pathology Testosterone / blood |
| Chemical | |
Reg. No./Substance:
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58-22-0/Testosterone; EC 3.4.15.1/Peptidyl-Dipeptidase A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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