| Expression of telomerase reverse transcriptase subunit (TERT) and telomere sizing in pig ovarian follicles. | |
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MedLine Citation:
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PMID: 16400001 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase is crucial for chromosome stability because it maintains telomere length. Little is known about telomerase in ovarian follicles, where an intense cell division is crucial to sustain estrous cycle and to drive oocyte development. The present research was performed to detect, by immunohistochemistry, the distribution of telomerase catalytic subunit (TERT) during folliculogenesis and to study the effect of TERT expression on telomeres. To this aim, telomere length has been measured on fluorescence in situ hybridization (FISH)-processed sections either in follicular or in germ cells. In primary and preantral follicles, TERT was observed in granulosa and in germ cells, with a typical nuclear location. During antral differentiation, only somatic cells close to the antrum (antral layer) and cumulus cells maintained TERT expression. The relative oocytes located TERT in the ooplasm independent from the process of meiotic maturation. FISH results indicate that a correlation exists between TERT expression and telomere size. In fact, progressively bigger telomeres were observed from preantral to antral follicles where longer structures were recorded in cells of the cumulus oophorus and of the antral layer than those of the basal one. Stable and elongated telomeres were detected in fully grown oocytes that lost the functional TERT distribution within the nucleus. |
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Authors:
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Valentina Russo; Paolo Berardinelli; Alessandra Martelli; Oriana Di Giacinto; Delia Nardinocchi; Donatella Fantasia; Barbara Barboni |
Publication Detail:
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Type: Journal Article Date: 2006-01-06 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 54 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-20 Completed Date: 2006-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 443-55 Citation Subset: IM |
Affiliation:
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Dipartimento di Scienze Biomediche Comparate, Università degli Studi di Teramo, 64100 Teramo, Italy. vrusso@unite.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Compartmentation DNA-Binding Proteins / biosynthesis* Female Immunohistochemistry In Situ Hybridization, Fluorescence Oocytes / metabolism, ultrastructure Ovarian Follicle / enzymology*, ultrastructure Protein Subunits / metabolism Swine Telomerase / biosynthesis* Telomere / ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Protein Subunits; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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