Document Detail


Expression of telomerase reverse transcriptase in adult goldfish retina.
MedLine Citation:
PMID:  17873300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Telomerase, a specialized reverse transcriptase that maintains telomere during cell division, is commonly associated with cell proliferation. Increasing evidence suggests that telomerase may bear functions other than telomere elongation. We investigated whether telomerase is expressed in the continuously growing goldfish retina. Telomeric repeat amplification protocol (TRAP) assay reveals telomerase activity in goldfish retina. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot show that telomerase catalytic subunit (TERT) is expressed at both mRNA and protein levels. Localization of TERT by immunohistochemistry indicates prominent expression of TERT in the outer nuclear layer, the inner nuclear layer, and, in a small population of cells, in the ganglion cell layer. Coexpression of TERT with proliferative cell nuclear antigen (PCNA) immunoreactivity is found in rod progenitor cells. These results suggest the role of telomerase in vertebrate central nervous system (CNS) other than telomere maintenance, such as regulation of cell cycle progression and maintenance of retinal cell phenotypes.
Authors:
Benson W M Lau; George S W Tsao; K F So; Henry K Yip
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  32     ISSN:  0895-8696     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  2007  
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  160-7     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Blotting, Western
Consensus Sequence
Gene Amplification
Goldfish / genetics
Molecular Sequence Data
Protein Biosynthesis
Repetitive Sequences, Amino Acid
Retina / enzymology*
Reverse Transcriptase Polymerase Chain Reaction
Telomerase / genetics*
Chemical
Reg. No./Substance:
EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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