| Expression of telomerase reverse transcriptase in adult goldfish retina. | |
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MedLine Citation:
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PMID: 17873300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase, a specialized reverse transcriptase that maintains telomere during cell division, is commonly associated with cell proliferation. Increasing evidence suggests that telomerase may bear functions other than telomere elongation. We investigated whether telomerase is expressed in the continuously growing goldfish retina. Telomeric repeat amplification protocol (TRAP) assay reveals telomerase activity in goldfish retina. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot show that telomerase catalytic subunit (TERT) is expressed at both mRNA and protein levels. Localization of TERT by immunohistochemistry indicates prominent expression of TERT in the outer nuclear layer, the inner nuclear layer, and, in a small population of cells, in the ganglion cell layer. Coexpression of TERT with proliferative cell nuclear antigen (PCNA) immunoreactivity is found in rod progenitor cells. These results suggest the role of telomerase in vertebrate central nervous system (CNS) other than telomere maintenance, such as regulation of cell cycle progression and maintenance of retinal cell phenotypes. |
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Authors:
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Benson W M Lau; George S W Tsao; K F So; Henry K Yip |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of molecular neuroscience : MN Volume: 32 ISSN: 0895-8696 ISO Abbreviation: J. Mol. Neurosci. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-09-17 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9002991 Medline TA: J Mol Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 160-7 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Blotting, Western Consensus Sequence Gene Amplification Goldfish / genetics Molecular Sequence Data Protein Biosynthesis Repetitive Sequences, Amino Acid Retina / enzymology* Reverse Transcriptase Polymerase Chain Reaction Telomerase / genetics* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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