| Expression of surfactant protein D in human corneal epithelial cells is upregulated by Pseudomonas aeruginosa. | |
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MedLine Citation:
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PMID: 18657106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We reported previously that surfactant protein D (SP-D) was present in human tears and corneal epithelial cells, and that it contributed to tear fluid protection of those cells against Pseudomonas aeruginosa invasion. This suggested a role in ocular innate immunity. Here, we explored the effects of bacterial challenge on SP-D expression by human corneal epithelial cells. Results showed that these cells produced and secreted SP-D constitutively in culture, and that production (mRNA, protein) and secretion of SP-D were upregulated after exposure to heat-killed P. aeruginosa or to purified flagellin or lipopolysaccharide. To begin exploring the mechanism for flagellin-mediated SP-D induction, cells were exposed to purified flagellin or flagellin mutated in the TLR-5-binding domain (L94A, L88A) which reduces IL-8 secretion by A549 respiratory cells. Mutated flagellin did not upregulate IL-8 expression in corneal epithelial cells, but did induce SP-D responses. Mitogen-activated protein kinase inhibitors, especially the JNK inhibitor SP600125, reduced secretion of SP-D, but not production, in the presence of P. aeruginosa. These data show that while SP-D and IL-8 corneal responses are each induced by P. aeruginosa or its antigens, they can involve different regions of the same ligand. The data suggest that separate mechanisms may regulate SP-D secretion and production by human corneal epithelia. |
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Authors:
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Minjian Ni; Connie Tam; Amrisha Verma; Reuben Ramphal; Samuel Hawgood; David J Evans; Suzanne M J Fleiszig |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-07-22 |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 54 ISSN: 0928-8244 ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-12-03 Completed Date: 2009-04-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 177-84 Citation Subset: IM |
Affiliation:
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School of Optometry, University of California, Berkeley, CA 94720, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Antigens, Bacterial / genetics, metabolism Cell Line, Transformed Enzyme Inhibitors / pharmacology Epithelium, Corneal / immunology*, metabolism*, microbiology, secretion Flagellin / genetics, immunology Gene Expression Regulation Humans Interleukin-8 / metabolism Ligands Lipopolysaccharides / immunology Point Mutation Pseudomonas aeruginosa / genetics, immunology* Pulmonary Surfactant-Associated Protein D / genetics, metabolism* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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AI47852/AI/NIAID NIH HHS; EY1121/EY/NEI NIH HHS; HL24075/HL/NHLBI NIH HHS; HL58047/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Enzyme Inhibitors; 0/Interleukin-8; 0/Ligands; 0/Lipopolysaccharides; 0/Pulmonary Surfactant-Associated Protein D; 12777-81-0/Flagellin |
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