Document Detail

Expression of surfactant protein D in human corneal epithelial cells is upregulated by Pseudomonas aeruginosa.
MedLine Citation:
PMID:  18657106     Owner:  NLM     Status:  MEDLINE    
We reported previously that surfactant protein D (SP-D) was present in human tears and corneal epithelial cells, and that it contributed to tear fluid protection of those cells against Pseudomonas aeruginosa invasion. This suggested a role in ocular innate immunity. Here, we explored the effects of bacterial challenge on SP-D expression by human corneal epithelial cells. Results showed that these cells produced and secreted SP-D constitutively in culture, and that production (mRNA, protein) and secretion of SP-D were upregulated after exposure to heat-killed P. aeruginosa or to purified flagellin or lipopolysaccharide. To begin exploring the mechanism for flagellin-mediated SP-D induction, cells were exposed to purified flagellin or flagellin mutated in the TLR-5-binding domain (L94A, L88A) which reduces IL-8 secretion by A549 respiratory cells. Mutated flagellin did not upregulate IL-8 expression in corneal epithelial cells, but did induce SP-D responses. Mitogen-activated protein kinase inhibitors, especially the JNK inhibitor SP600125, reduced secretion of SP-D, but not production, in the presence of P. aeruginosa. These data show that while SP-D and IL-8 corneal responses are each induced by P. aeruginosa or its antigens, they can involve different regions of the same ligand. The data suggest that separate mechanisms may regulate SP-D secretion and production by human corneal epithelia.
Minjian Ni; Connie Tam; Amrisha Verma; Reuben Ramphal; Samuel Hawgood; David J Evans; Suzanne M J Fleiszig
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-07-22
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  54     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-12-03     Completed Date:  2009-04-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  177-84     Citation Subset:  IM    
School of Optometry, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Analysis of Variance
Antigens, Bacterial / genetics,  metabolism
Cell Line, Transformed
Enzyme Inhibitors / pharmacology
Epithelium, Corneal / immunology*,  metabolism*,  microbiology,  secretion
Flagellin / genetics,  immunology
Gene Expression Regulation
Interleukin-8 / metabolism
Lipopolysaccharides / immunology
Point Mutation
Pseudomonas aeruginosa / genetics,  immunology*
Pulmonary Surfactant-Associated Protein D / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Antigens, Bacterial; 0/Enzyme Inhibitors; 0/Interleukin-8; 0/Ligands; 0/Lipopolysaccharides; 0/Pulmonary Surfactant-Associated Protein D; 12777-81-0/Flagellin

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