Document Detail

Expression and subcellular localization of TLR-4 in term and first trimester human placenta.
MedLine Citation:
PMID:  16338476     Owner:  NLM     Status:  MEDLINE    
Toll-like receptor 4 (TLR-4) mediates Gram-negative bacterial-induced inflammatory responses, including production of pro-inflammatory cytokines. Maternal infection and inflammation play an important role in preterm birth and neonatal brain damage. The localization of placental TLR-4 as well as changes during normal gestation are critical issues in understanding the role of toll-like receptors in defending the placento-fetal unit from maternal infection. We therefore investigated, by immunohistochemistry (IHC) and Western blot, the subcellular localization of TLR-4 in first trimester and term human placenta. In both term placenta (n=4) and first trimester placenta villous samples (n=5), immunoreactivity for TLR-4 was found in the cytoplasm of the syncytiotrophoblast, with darker staining in some areas of the maternal facing plasma membrane (MVM). In addition, TLR-4 was found to be expressed in the first trimester cytotrophoblast cells. Using Western blot analysis, TLR-4 was identified in both placental homogenates and isolated MVM and the fetal facing basal membrane (BM). TLR-4 expression in MVM was significantly higher in term (n=9) as compared to first trimester (n=2) samples. We have shown for the first time that the subcellular localization of TLR-4 in term placenta is preferentially in the MVM compared to BM. The MVM is continuously bathed in maternal blood, suggesting that from this vantage point TLR-4 can initiate a rapid response to maternal bacterial infection.
E C E Beijar; C Mallard; T L Powell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Placenta     Volume:  27     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2006 Feb-Mar
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  322-6     Citation Subset:  IM    
Perinatal Center, Department of Physiology, Göteborg University, 405 30 Göteborg, Sweden.
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MeSH Terms
Cell Membrane / chemistry*
Placenta / chemistry,  cytology,  metabolism*
Pregnancy Trimester, First / metabolism*
Toll-Like Receptor 4 / analysis*,  metabolism*
Reg. No./Substance:
0/TLR4 protein, human; 0/Toll-Like Receptor 4

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