| Expression and subcellular localization of TLR-4 in term and first trimester human placenta. | |
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MedLine Citation:
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PMID: 16338476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toll-like receptor 4 (TLR-4) mediates Gram-negative bacterial-induced inflammatory responses, including production of pro-inflammatory cytokines. Maternal infection and inflammation play an important role in preterm birth and neonatal brain damage. The localization of placental TLR-4 as well as changes during normal gestation are critical issues in understanding the role of toll-like receptors in defending the placento-fetal unit from maternal infection. We therefore investigated, by immunohistochemistry (IHC) and Western blot, the subcellular localization of TLR-4 in first trimester and term human placenta. In both term placenta (n=4) and first trimester placenta villous samples (n=5), immunoreactivity for TLR-4 was found in the cytoplasm of the syncytiotrophoblast, with darker staining in some areas of the maternal facing plasma membrane (MVM). In addition, TLR-4 was found to be expressed in the first trimester cytotrophoblast cells. Using Western blot analysis, TLR-4 was identified in both placental homogenates and isolated MVM and the fetal facing basal membrane (BM). TLR-4 expression in MVM was significantly higher in term (n=9) as compared to first trimester (n=2) samples. We have shown for the first time that the subcellular localization of TLR-4 in term placenta is preferentially in the MVM compared to BM. The MVM is continuously bathed in maternal blood, suggesting that from this vantage point TLR-4 can initiate a rapid response to maternal bacterial infection. |
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Authors:
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E C E Beijar; C Mallard; T L Powell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Placenta Volume: 27 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2006 Feb-Mar |
Date Detail:
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Created Date: 2005-12-12 Completed Date: 2006-03-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 322-6 Citation Subset: IM |
Affiliation:
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Perinatal Center, Department of Physiology, Göteborg University, 405 30 Göteborg, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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chemistry* Female Humans Placenta / chemistry, cytology, metabolism* Pregnancy Pregnancy Trimester, First / metabolism* Toll-Like Receptor 4 / analysis*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/TLR4 protein, human; 0/Toll-Like Receptor 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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