| Expression and subcellular localization of ORC1 in Leishmania major. | |
| | |
MedLine Citation:
|
PMID: 18680728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mechanism of DNA replication is highly conserved in eukaryotes, with the process being preceded by the ordered assembly of pre-replication complexes (pre-RCs). Pre-RC formation is triggered by the association of the origin replication complex (ORC) with chromatin. Leishmania major appears to have only one ORC ortholog, ORC1. ORC1 in other eukaryotes is the largest of the ORC subunits and is believed to play a significant role in modulating replication initiation. Here we report for the first time, the cloning of ORC1 from L. major, and the analysis of its expression in L. major promastigotes. In human cells ORC1 levels have been found to be upregulated in G1 and subsequently degraded, thus playing a role in controlling replication initiation. We examine the subcellular localization of L. major ORC1 in relation to the different stages of the cell cycle. Our results show that, unlike what is widely believed to be the case with ORC1 in human cells, ORC1 in L. major is nuclear at all stages of the cell cycle. |
| | |
Authors:
|
Diwakar Kumar; Agnideep Mukherji; Swati Saha |
Related Documents
:
|
16274748 - The cell cycle as a therapeutic target for alzheimer's disease. 8985018 - Circadian rhythms in rapidly dividing cyanobacteria. 10950718 - Cell cycle. replication meets cohesion. . 16534938 - Cell cycle regulation in marine synechococcus sp. strains. 8408648 - Infection of human synovial cells by human t cell lymphotropic virus type i. proliferat... 16274748 - The cell cycle as a therapeutic target for alzheimer's disease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-03 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 375 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-09-01 Completed Date: 2008-09-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 74-9 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Division Cloning, Molecular DNA Replication Humans Leishmania major / cytology, genetics, metabolism* Origin Recognition Complex / genetics, metabolism* Protozoan Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Origin Recognition Complex; 0/Protozoan Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overexpression of Arabidopsis ZEP enhances tolerance to osmotic stress.
Next Document: Contribution of alpha4beta1 integrin to the antiallergic effect of levocabastine.