Document Detail


Expression of stress-related genes in a cadmium-resistant A549 human cell line.
MedLine Citation:
PMID:  16020198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to explain the basis for Cd-acquired tolerance of A549 cells cultured in the presence of Cd. Thirty-day exposure of cultured human pneumocytes (A549 cell line) to 10 microM Cd was previously found to induce an acquired resistance persisting over several weeks of culture. Moreover, these Cd-resistant cells (R-cells) were found to proliferate faster than controls. No difference was found between R-cells and control cells (S-cells) concerning the basal and Cd-induced level of metallothioneins expression. However, after exposure to Cd, cell glutathione levels were unchanged in R-cells while they were either increased (at 10 microM Cd) or decreased (at 25 microM Cd) in S-cells. cDNA array analysis showed that genes encoding for (GPx1) glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase were similarly expressed in R- and S-cells, whereas the gene of (GPx2) glutathione peroxidase was overexpressed in R-cells. Most genes encoding stress proteins were similarly expressed, except for HSP27 and GRP94 genes, which were respectively under- (ratio 0.5 +/- 0.1) and over- (1.8 +/- 0.5) expressed in R-cells. Acute exposure to Cd was found to trigger the upregulation of genes encoding the chaperone proteins HSP90A, HSP27, HSP40, GRP78, HSP72, and HO-1 in S-cells. In R-cells, only HO-1 and HSP72 were overexpressed but at a lower level. This suggests that the Cd-related adverse conditions, leading to protein misfolding, are lowered in R-cells. It is likely that the upregulation of GPx2 in R-cells leads to a higher antioxidant defense in these cells.
Authors:
Françoise Croute; Bernadette Beau; Jean-Claude Murat; Christian Vincent; Hiroyoshi Komatsu; Fumiya Obata; Jean-Pierre Soleilhavoup
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  68     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-19     Completed Date:  2005-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  703-18     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire et Pollution, Faculté de Médecine Toulouse-Purpan, Toulouse, France. croute@cict.fr
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma
Cadmium / toxicity*
Gene Expression Profiling
Glutathione / metabolism*
Humans
Oligonucleotide Array Sequence Analysis
Oxidative Stress / drug effects,  genetics*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
70-18-8/Glutathione; 7440-43-9/Cadmium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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