Document Detail


Expression of statin, a non-proliferation-dependent nuclear protein, in the postnatal rat brain: evidence for substantial retention of neuroglial proliferative capacity with aging.
MedLine Citation:
PMID:  2271926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Statin is a 57 kDa protein expressed in nuclei of reversibly and irreversibly growth-arrested (Go-phase) cells. In this report, immunohistochemical localization of statin in the developing and aging rat brain was achieved using the monoclonal antibody, S-44. On postnatal day 2, post-migratory neurons in the developing cerebral cortex were statin-positive. Many statin-negative cells were observed in the lateral subependymal zone of the lateral ventricle. By postnatal day 10, most neuronal nuclei were statin-positive although small numbers of statin-negative neurons were still encountered in the lateral subependymal zone and hippocampal dentate gyrus. At 3, 18 and 33 months, all neuronal nuclei surveyed were statin-positive. These results support the contention that, save for the postnatal persistence of 'germinal zones' such as the subependymal region and dentate gyrus, neuronal proliferation in the rat is largely completed by the time of birth. In striking contrast to neuronal statin expression, a significant fraction of neuroglia in both grey and white matter remains statin-negative at all ages examined. In the corpus callosum, 33.2%, 34.0% and 34.7% of glial nuclei were statin-negative at 3, 18 and 33 months, respectively. These findings indicate that: (i) even in senescent brain, the cycling (statin-negative) glial pool is substantially larger than previously surmised from S-phase labeling experiments; and (ii) during aging, the ratio of noncycling-to-cycling neuroglia remains very tightly regulated. Examination of other non-neuronal cell types revealed that most, if not all, ependymal and choroid plexus epithelial cells were statin-positive in the neonatal and adult brains in keeping with the predominantly prenatal proliferation of these tissues. Our results indicate that statin immunolabeling using the S-44 antibody is a powerful technique for the in situ identification of non-proliferating cells in the developing and aging nervous system.
Authors:
H M Schipper; E Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  528     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-02-28     Completed Date:  1991-02-28     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  250-8     Citation Subset:  IM    
Affiliation:
Department of Neurology, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aging / pathology*
Animals
Animals, Newborn / metabolism
Brain Chemistry / physiology*
Cell Count
Cell Cycle Proteins
Cell Division / physiology
Immunohistochemistry
Male
Nerve Tissue Proteins / analysis*
Neuroglia / cytology*
Nuclear Proteins / analysis*
Proteins / analysis*
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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