Document Detail

Expression of the spr1 gene in cultured tracheal epithelial cells and its regulation by retinoids before and after confluence.
MedLine Citation:
PMID:  8600151     Owner:  NLM     Status:  MEDLINE    
The absence of vitamin A or vitamin A derivatives in culture media promotes squamous cell differentiation of tracheobronchial epithelial cells. This is especially true for the expression of a small proline-rich protein (20K; 98 amino acids) in pig trachea epithelial cells. Multigene families encode different small proline-rich proteins in different species, and these proteins are possible markers for squamous cell differentiation. 20K mRNA and 20K protein were detected in cells within 4 and 5 days in culture, respectively, when cells reached about 50% confluence, and expression increase 12-fold during cell proliferation until cells reached 100% confluence. Arotinoid (10(-9)M), a synthetic retinoid, essentially totally inhibited expression of 20K mRNA in proliferating tracheobronchial cells within 3 days of treatment while 20K protein levels were only decreased 4-fold after 5 days. However, if cells were exposed to arotinoid 3 days after reaching confluent growth, the levels of either 20K mRNA or 20K protein were unchanged. Cells exposed to arotinoid from the onset of culturing, and then removal of the retinoid from proliferating cells resulted in the expression of 20K mRNA and protein after 4 and 5 days as observed previously. 20K mRNA was not detected in cells that had been continuously exposed to arotinoid from the start of culture until 3 days post confluence, even 10 days following removal of arotinoid. Our results strongly suggest that the growth phase and state of cell differentiation greatly affect the response of these epithelial cells to vitamin A derivatives.
J Tesfaigzi; D M Carlson
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  166     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-05-02     Completed Date:  1996-05-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  480-6     Citation Subset:  IM    
Inhalation Toxicology Research Institute, Albuquerque, New Mexico, 87185, USA.
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MeSH Terms
Amino Acid Sequence
Cell Division
Cells, Cultured
Cornified Envelope Proline-Rich Proteins
Epithelial Cells
Epithelium / metabolism
Gene Expression Regulation / drug effects*
Membrane Proteins
Molecular Sequence Data
Protein Biosynthesis
Proteins / genetics*
RNA, Messenger / biosynthesis
Retinoids / pharmacology*
Trachea / cytology,  metabolism*
Grant Support
Reg. No./Substance:
0/Cornified Envelope Proline-Rich Proteins; 0/Membrane Proteins; 0/Proteins; 0/RNA, Messenger; 0/Retinoids

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