Document Detail

Expression of ski in the granulosa cells of atretic follicles in the rat ovary.
MedLine Citation:
PMID:  16926528     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to locate Ski protein, a product of cellular protooncogene c-ski, in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin. These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggests that Ski plays a role in apoptosis of granulosa cells during follicular atresia.
Hyun Kim; Keitaro Yamanouchi; Masugi Nishihara
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Publication Detail:
Type:  Journal Article     Date:  2006-08-23
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  52     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-01-15     Completed Date:  2007-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  715-21     Citation Subset:  IM    
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Japan.
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MeSH Terms
DNA-Binding Proteins / biosynthesis*,  genetics
Estradiol / blood
Estrous Cycle / genetics,  physiology
Follicular Atresia / genetics,  metabolism*
Gene Expression
Granulosa Cells / cytology,  metabolism*
In Situ Nick-End Labeling
Proliferating Cell Nuclear Antigen / metabolism
Proto-Oncogene Proteins / biosynthesis*,  genetics
RNA, Messenger / biosynthesis,  genetics
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proliferating Cell Nuclear Antigen; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 50-28-2/Estradiol

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