| Expression of a single betaalpha chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity. | |
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MedLine Citation:
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PMID: 11306169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Equine chorionic gonadotropin (eCG) is a heavily glycosylated glycoprotein composed of non-covalently linked alpha- and beta-subunits. eCG possesses the particularity to bind to both LH and FSH receptors in species other than horses and to have a prolonged plasma half-life. All these properties make it of utmost interest for livestock fertilization program. Up to now, the only source of eCG is the serum of pregnant mare. Rabbit mammary gland is considered as a system able to produce recombinant glycoproteins in sufficient quantity for pharmaceutical use. Here we described the production of a recombinant single betaalpha chain of eLH/CG in the milk of transgenic rabbit. The construction of a single-chain permits to by-pass the problem of association-dissociation of the subunits. This recombinant hormone is greatly expressed (21.7 mg/l) and presents similar in vitro LH and FSH bioactivities. However, betaalphaeLH/CG shows an extremely rapid clearance (approximately 10 min), which could explain the absence of in vivo biological activity. So the rabbit mammary gland is not appropriate for the production of a recombinant active eLH/CG. |
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Authors:
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C Galet; C M Le Bourhis; M Chopineau; G Le Griec; A Perrin; T Magallon; J Attal; C Viglietta; L M Houdebine; F Guillou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 174 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-04-18 Completed Date: 2001-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 31-40 Citation Subset: IM |
Affiliation:
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Laboratoire de Physiologie de la Reproduction et des Comportements, UMR INRA-CNRS Université de Tours, 37380, Nouzilly, France. galet@tours.inra.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified / metabolism Breast / metabolism Cell Line Chorionic Gonadotropin / biosynthesis, genetics*, pharmacology DNA, Complementary / metabolism Female Horses Luteinizing Hormone / biosynthesis, genetics*, pharmacology Male Milk / chemistry* Protein Subunits Rabbits Rats Receptors, LH / metabolism Recombinant Proteins / biosynthesis, genetics, pharmacology Testosterone / agonists Transgenes |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/DNA, Complementary; 0/Protein Subunits; 0/Receptors, LH; 0/Recombinant Proteins; 58-22-0/Testosterone; 9002-67-9/Luteinizing Hormone |
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