| Expression and significance of cell immunohistochemical markers (HHF-35, CD-31, Bcl-2, P-53 and apopDETEC) in hypertrophic cardiomyopathy. | |
| | |
MedLine Citation:
|
PMID: 14702165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
There are several hypotheses concerning the pathogenesis of hypertrophic cardiomyopathy (genetic, ischaemic, immune, inflammatory and apoptosis induction). We have studied three types of cardiomyopathy in order to observe the expression and assess the significance of different immunohistochemical markers (muscular actin, CD-31, proliferation cell nuclear antigen -PCNA-, Ki-67, and markers related with programmed cell death, bcl-2, p-53 and apopDETEC). We studied different microscopic (haematoxylin-eosin and Masson's thrichrome) and immunohistochemical parameters (streptavidin-biotin-peroxidase and "in situ" hybridisation) of forty cases: ten each of hypertensive hypertrophic cardiomyopathy, essential hypertrophic cardiomyopathy, hypertrophic cardiomyopathy in patients treated with chemotherapy and morphologically "normal" hearts. Our findings point to an absence of structural marker expression (actin and CD-31) in cases of hypoxic damage. The distribution and intensity of apoptosis markers, a seen by "in situ" hybridisation were irregular, and the rest of the markers studied showed negative results, with the exception of acridin orange (a marker of hypoxic damage). In our opinion, the above immunohistochemical markers, especially actin and CD-31, could be used for differentiating hypoxic lesions in these three types of cardiomyopathy. Moreover, it is difficult to know the significance of the apoptosis markers, because the autolysis process produces cross reactions with false positive results. We think that there is a need for new studies on DNA breakdown processes during the post-mortem interval. To avoid autolysis problems the post-mortem material needs to be as fresh as possible. |
| | |
Authors:
|
F Martínez-Díaz; M Bernal-Gilar; M Gómez-Zapata; A Luna |
Related Documents
:
|
10506215 - Linkage of the bh4 domain of bcl-2 and the nuclear factor kappab signaling pathway for ... 16214115 - Modulation of mdr1 gene expression in multidrug resistant mcf7 cells by low concentrati... 10779415 - Specific and rapid induction of the proapoptotic protein hrk after growth factor withdr... 15161035 - Effect of octreotide on apoptosis-related proteins in rat kupffer cells: a possible ant... 10810285 - Localization of type 1 17beta-hydroxysteroid dehydrogenase mrna and protein in syncytio... 15086535 - Androgen regulation of the human hair follicle: the type i hair keratin hha7 is a direc... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Histology and histopathology Volume: 19 ISSN: 0213-3911 ISO Abbreviation: Histol. Histopathol. Publication Date: 2004 Jan |
Date Detail:
|
Created Date: 2003-12-31 Completed Date: 2004-08-30 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
|
Languages: eng Pagination: 9-14 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Medicine School of Murcia, University of Murcia, Murcia, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acridine Orange
/
analysis,
metabolism Actins / analysis, metabolism Adult Aged Antigens, CD31 / analysis, metabolism Apoptosis Biological Markers / analysis* Cardiomyopathy, Hypertrophic / metabolism*, pathology DNA Damage Heart Ventricles / cytology, pathology Humans Immunohistochemistry In Situ Hybridization Ki-67 Antigen / analysis, metabolism Myocardium / cytology, pathology* Myocytes, Cardiac / metabolism, pathology Organ Size Proliferating Cell Nuclear Antigen / analysis, metabolism Proto-Oncogene Proteins c-bcl-2 / analysis, metabolism Tumor Suppressor Protein p53 / analysis, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/Antigens, CD31; 0/Biological Markers; 0/Ki-67 Antigen; 0/Proliferating Cell Nuclear Antigen; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 65-61-2/Acridine Orange |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distributional map of the terminal and sub-terminal sugar residues of the glycoconjugates in the pre...
Next Document: Pathological change of articular cartilage in the mandibular head treated with immunosuppressant FK ...