Document Detail

Expression and secretion of neuroleukin/phosphohexose isomerase/maturation factor as autocrine motility factor by tumor cells.
MedLine Citation:
PMID:  9635595     Owner:  NLM     Status:  MEDLINE    
The results obtained from fragmented protein microsequencing have suggested that autocrine motility factor (AMF), a tumor-secreted Mr 55,000 cytokine that regulates cell motility in vitro as well as invasion and metastasis in vivo, is the neuroleukin (NLK)/phosphohexose isomerase (PHI)/maturation factor (MF) polypeptide. Here, we cloned, sequenced, and studied the expression, secretion, and distribution of AMF/NLK/PHI/MF in neoplastic and their normal counterpart cells. Although both normal and neoplastic cells express the gene product, overexpression associated with selective secretion of the protein was observed only in tumor cells. The cDNA sequences of AMF/NLK/PHI/MF found in both human cancer and normal cells were found to be identical, suggesting that its secretion by neoplastic cells is independent of mutation or alternative splicing. Immunohistochemical visualization has depicted AMF/NLK/PHI/MF to be localized into tubular-like vesicles, diffusely distributed throughout the cytoplasm and not colocalized with any particular cytoskeletal network. Confocal microscopic imaging had shown a partial colocalization between AMF and its receptor (Mr 78,000 glycoprotein), especially on the malignant cell surface periphery. The results suggest that extracellular AMF activity may be a result of the product of intracellular cleavage of a precursor polypeptide, which is overexpressed and selectively secreted through a nonclassical secretory mechanism by neoplastic cells.
Y Niinaka; S Paku; A Haga; H Watanabe; A Raz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  58     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-01     Completed Date:  1998-07-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2667-74     Citation Subset:  IM    
Metastasis Research Program, Karmanos Cancer Institute, Detroit, Michigan 48201, USA.
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MeSH Terms
3T3 Cells
Amino Acid Sequence
Autocrine Motility Factor / genetics*,  metabolism
Base Sequence
Fluorescent Antibody Technique
Glucose-6-Phosphate Isomerase / metabolism*
Maturation-Promoting Factor / metabolism*
Molecular Sequence Data
Neoplasm Proteins / metabolism*
Neuroleukin / metabolism*
Tumor Cells, Cultured / metabolism
Grant Support
Reg. No./Substance:
0/Autocrine Motility Factor; 0/Neoplasm Proteins; 0/Neuroleukin; EC Factor; EC Isomerase

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