Document Detail


Expression and secretion of antiviral factors by trophoblast cells following stimulation by the TLR-3 agonist, Poly(I : C).
MedLine Citation:
PMID:  16751646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: During pregnancy, the placenta may become exposed to micro-organisms, such as viruses, which may pose a substantial threat to the embryo/fetus well-being. Recent insight into the immunological capabilities of the trophoblast suggests that the placenta may function as an active barrier by recognizing and responding to pathogens through Toll-like receptors (TLRs). METHODS: The objective of this study was to determine whether the engagement of TLR-3 with viral dsRNA by first-trimester trophoblast could induce the production of factors necessary to generate an antiviral response. Therefore, trophoblast cells were exposed to the TLR-3 agonist, Poly(I : C). RESULTS: We report that following stimulation with Poly(I : C), first-trimester trophoblast cells produce interferon beta (IFNbeta) and secretory leukocyte protease inhibitor (SLPI), as well as the intracellular factors 2',5'-oligoadenylate synthetase (OAS), Myxovirus-resistance A (MxA) and apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G). This response is TLR-3 specific because the TLR-4 ligand, lipopolysaccharide (LPS), had no effect on the production of these antimicrobial factors. Furthermore, we describe a positive feedback mechanism in which IFNbeta enhances the antiviral response by promoting the production of OAS, MxA and APOBEC3G. CONCLUSIONS: These findings suggest that trophoblast cells are able to recognize and specifically respond to viral products in a highly regulated fashion and that the placenta may be pivotal in the control of viral infections at the maternal-fetal interface.
Authors:
Vikki M Abrahams; Todd M Schaefer; John V Fahey; Irene Visintin; Jacqueline A Wright; Paulomi B Aldo; Roberto Romero; Charles R Wira; Gil Mor
Related Documents :
19478466 - Detection of fetal cells in the maternal kidney during gestation in the mouse.
12434116 - Large granular lymphocytes (lgls) activity in women with spontaneous abortions during t...
8388816 - Spatial and temporal expression of connexin26 and connexin43 in rat endometrium during ...
19741206 - Pregnancy enhances sustained ca2+ bursts and endothelial nitric oxide synthase activati...
17805206 - Prenatal stress and mixed-handedness.
12680676 - Interpreting results from field deployments of semipermeable membrane devices.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2006-06-03
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  21     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2007-01-30     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  2432-9     Citation Subset:  IM    
Affiliation:
Department of Obstetrics, Gynecology & Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antiviral Agents / pharmacology*
Apolipoproteins B / chemistry
Female
Humans
Ligands
Lipopolysaccharides / metabolism
Poly I-C / pharmacology*
Pregnancy
Pregnancy Trimester, First
RNA, Double-Stranded / chemistry
Reverse Transcriptase Polymerase Chain Reaction
Toll-Like Receptor 3 / agonists*
Trophoblasts / metabolism*
Grant Support
ID/Acronym/Agency:
2N01HD23342/HD/NICHD NIH HHS; AI51877/AI/NIAID NIH HHS; R01HD049446-01/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Apolipoproteins B; 0/Ligands; 0/Lipopolysaccharides; 0/RNA, Double-Stranded; 0/Toll-Like Receptor 3; 24939-03-5/Poly I-C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Monitoring reproductive health in Europe--what are the best indicators of reproductive health?
Next Document:  Cumulative pregnancy rates after three cycles of minimal stimulation IVF and results according to su...