| Expression of the rubber-like protein, resilin, in developing and functional insect cuticle determined using a Drosophila anti-rec 1 resilin antibody. | |
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MedLine Citation:
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PMID: 22275226 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: The natural elastomeric protein, insect resilin, is the most efficient elastic material known, used to store energy for jumping and flight in a variety of insects. Here, an antibody to recombinant Drosophila melanogaster pro-resilin is used to examine resilin expression in Drosophila and a wider range of insects. Results: Immunostaining of Drosophila embryos reveals anti-resilin reactivity in epidermal patches that exhibit a dynamic spatial and temporal expression through late embryogenesis. Resilin is also detected in stretch receptors in the embryo. In developing adult Drosophila, resilin pads are described at the base of wings and at the base of flexible sensory hairs in pupae. Resilin is also detected in embryos of the tephritid fruitfly, Bactrocera tryoni, and two well-known concentrations of insect resilin: the flight muscle tendon of the dragonfly and the pleural arch of the flea. Conclusions: The anti-Rec1 antibody antibody developed using Drosophila pro-resilin as antigen is cross-reactive and is useful for detection of resilin in diverse insects. For the first time, resilin expression has been detected during embryogenesis, revealing segmental patches of resilin in the developing epidermis of Drosophila. Developmental Dynamics 241:333-339, 2012. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Darren C C Wong; Roger D Pearson; Christopher M Elvin; David J Merritt |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 241 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 333-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Department of Molecular, Cell and Developmental Biology, UCLA, Los Angeles, California. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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